Št. zadetkov: 24
Izvirni znanstveni članek
Oznake:
mikrobiologija;metode;elektroforeza;DGGE;
Denaturant gradient gel electrophoresis (DGGE) enables insight into the diversity of the studied microbial communities on the basis of separation of PCR amplification products according to their nucelotide sequence composition. However, the success of the method is accompanied by the inherent appear ...
Leto:
2012
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
mikrobiologija;bakterije;arheje;metanogeneza;kunci;cekum;molekularna genetika;identifikacija;
During a comparison of 16S rDNA PCR-denaturant gradient gel electrophoresis (DGGE) profiles of methanogenic archea from rumen fluid, rabbit caecum and pig feces, a unique band common to all rabbit caecum samples was observed. DGGE profiling also showed that the methanogen community from the New Zeal ...
Leto:
2010
Vir:
Biotehniška fakulteta (UL BF)
Doktorska disertacija
Oznake:
Molekularna epidemiologija;listerioza – mikrobiologija;Listeria monocytogenes – izolacija in čiščenje – genetika;tipizacija na osnovi zaporedij več lokusov – metode;sekvenciranje celotnega genoma – metode;
Listerioza je resno obolenje ljudi in živali, ki ga povzroča bakterija Listeria monocytogenes. Sekvenciranje celotnih genomov (angl. whole-genome sequencing, WGS) postaja metoda izbora za epidemiološki nadzor listerioze, vključno s preiskavami izbruhov. Tipizacija izolatov ni sestavni del rutinskih ...
Leto:
2020
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
Paenibacillus larvae;American foulbrood;whole-genome sequencing;whole-genome multilocus sequence typing;core-genome multilocus sequence typing;whole-genome single nucleotide polymorphism;genotype;molecular sequence;
Paenibacillus larvae causes the American foulbrood (AFB), a highly contagious and devastating disease of honeybees. Whole-genome sequencing (WGS) has been increasingly used in bacterial pathogen typing, but rarely applied to study the epidemiology of P. larvae. To this end, we used 125 P. larvae gen ...
Leto:
2021
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
Microbiology - veterinary;Listeria monocytogenes - genetics;listeriosis;PFGE;MLST;population structure;cattle;
Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both animals and humans. Here, multilocus sequence typing (MLST) was used to characterize the genetic diversity of Listeria monocytogenes strains isolated from the natural environment and animal clinical cases ...
Leto:
2019
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
Whole genome sequencing;Methods;
An extensive source investigation was conducted on a dairy farm with neurolisteriosis and subclinical mastitis cases to identify infection source and potential transmission routes of Listeria monocytogenes.
Leto:
2019
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
epidemiology;veterinary;Listeria monocytogenes;outbreak;whole-genome sequencine;single nucleotide polymorphism;multilocus sequence typing;sheep;goats;
Listeria monocytogenes is the causative agent of listeriosis, a serious disease affecting both humans and animals. While listeriosis outbreaks in humans are commonly investigated in detail, routine typing of L. monocytogenes is generally not performed in animal outbreaks. Here, seven presumable list ...
Leto:
2020
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
antimicrobial susceptibility testing;epidemiology;methicillin-resistant Staphylococcus pseudintermedius (MRSP);small animal clinic;whole-genome sequencing (WGS);
Staphylococcus pseudintermedius is a common cause of skin and soft tissue infections in dogs but can also cause infections in cats and humans. The frequency of methicillin-resistant S. pseudintermedius (MRSP) strains is increasing worldwide. Here, we obtained 43 MRSP isolates from dogs (n = 41), one ...
Leto:
2021
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
Brucella suis;biovar 2;wildlife;whole-genome sequencing;
Brucella suis commonly infects swine but occasionally also other animal species and humans. Wild boars are the most important reservoir of B. suis biovar 2, continually infecting susceptible hosts through close contact. Nevertheless, the genetic diversity of B. suis in wildlife remains understudied. ...
Leto:
2023
Vir:
Veterinarska fakulteta (UL VF)
Izvirni znanstveni članek
Oznake:
Helicobacter;novel species;red fox;Vulpes vulpes;gastric mucosa;
Six Helicobacter- like isolates were recovered from 15 gastric mucosa samples of red foxes (Vulpes vulpes) shot by hunters in the surroundings of Ljubljana, Slovenia. Gram-negative, tightly coiled, intensely motile, 7-15 micro m long and 1 micro m wide bacteria grew on the biphasic blood agar plates ...
Leto:
2020
Vir:
Veterinarska fakulteta (UL VF)