Imobilizacija hrenove peroksidaze na maghemitne nanodelce, modificirane z aminosilanom in hitozanom

diplomsko delo univerzitetnega študijskega programa

Nataša Pezdevšek (Author), Maja Leitgeb (Mentor), Mateja Primožič (Co-mentor)

Abstract

Namen diplomske naloge je optimizacija procesnih pogojev za imobilizacijo encima hrenove peroksidaze na površinsko modificirane maghemitne nanodelce. Spreminjali smo masno razmerje med nosilcem in encimom, koncentracijo mrežnega povezovalca glutaraldehida in čas imobilizacije. Primerjali smo učinkovitost imobilizacije ter ohranjeno aktivnost encima, vezanega na aminosilanske in hitozanske maghemitne nanodelce. Maghemitne nanodelce smo sintetizirali s koprecipitacijo Fe2+ in Fe3+ ionov ob dodatku obarjalnega reagenta, 25 % amonijaka. Aminosilanske maghemitne nanodelce smo pripravili v dveh stopnjah. Najprej smo nanodelce prevlekli s funkcionalno plastjo silike (SiO2), nato pa še s plastjo aminosilana. Hitozanski maghemitni nanodelci so bili pripravljeni po treh različnih postopkih. Kljub dobri učinkovitosti imobilizacije, se v nobenem primeru ni ohranila visoka aktivnost encima. Najvišjo ohranjeno aktivnost imobiliziranega encima smo dosegli pri aminosilanskih nanodelcih, in sicer 2,06 % pri 65,76 % učinkovitosti imobilizacije. Prav tako je imobiliziran encim v primerjavi s prostim encimom pokazal nižjo stabilnost pri povišanem tlaku in temperaturi. Z zvišanjem temperature pri atmosferskem tlaku, se je aktivnost prostega in imobiliziranega encima izboljšala.

Keywords

imobilizacija encima;hrenova peroksidaza;maghemitni nanodelci;aminosilanski maghemitni nanodelci;hitozanski maghemitni nanodelci;učinkovitost imobilizacije;aktivnost encima;

Data

Language:	Slovenian
Year of publishing:	2012
Source:	Maribor
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[N. Pezdevšek]
UDC:	579.22(043.2)
COBISS:	16704278
Views:	2254
Downloads:	312
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Immobilization of herseradish peroxidase onto maghemite nanoparticles, modified with aminosilane and chitosan
Secondary abstract:	The purpose of the diploma thesis is optimization of process conditions for immobilization of horseradish peroxidase onto surface modified maghemite nanoparticles. By varying the mass ratio between carrier and enzyme, concentration of glutaraldehyde and duration time of immobilization, the comparison of the binding efficiency and residual activity of enzyme, immobilized onto maghemite nanoparticles coated by aminosilane and chitosan was done. Maghemite nanoparticles were synthetized by coprecipitation of Fe2+ and Fe3+ ions by adding an 25 % ammonia as precipitation agent. Aminosilane coated maghemite nanoparticles were prepared in two stages. First, maghemite nanoparticles were functionalized with silica (SiO2) and afterwards with aminosilane. Chitosan coated nanoparticles were prepared by three different procedures. Despite well binding efficiency, there were no high residual activities obtained. The highest activity preserved was in case of enzyme, immobilized onto aminosilane coated nanoparticles: 2,06 % at 65,76 % binding efficiency. Immobilized enzyme also demonstrated lower stability at higher pressure and temperature in comparison with free enzyme. Residual activity of free and immobilized enzyme increased by raising temperature at atmospheric conditions.
Secondary keywords:	enzyme immobilization;horseradish peroxidase;maghemite nanoparticles;aminosilane-coated maghemite nanoparticles;chitosan-coated maghemite nanoparticles;binding efficiency;anzyme activity;
URN:	URN:SI:UM:
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	XIII, 55 str.
Keywords (UDC):	mathematics;natural sciences;naravoslovne vede;matematika;biological sciences in general;biologija;microbiology;mikrobiologija;general microbiology;
ID:	1001165