Ivan Radosavljević (Author), Zlatko Šatović (Author), Jernej Jakše (Author), Branka Javornik (Author), Danijela Greguraš (Author), Marija Jug-Dujaković (Author), Zlatko Liber (Author)

Abstract

Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H$_E$) was compared to the expected genetic diversity at mutation-drift equilibrium (H$_{EQ}$) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.

Keywords

salvia;common sage;laminaceae;microsatellites;microsatellite markers;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 577.2:633.88
COBISS: 7252089 Link will open in a new window
ISSN: 1661-6596
Views: 1065
Downloads: 473
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Other data

Secondary language: Slovenian
Secondary keywords: navadni žajbelj;dalmatinski žajbelj;mikrosatelitski markerji;mikrosateliti;populacije;laminaceae;genetska pestrost;
Type (COBISS): Scientific work
Pages: str. 12082-12093
Volume: ǂVol. ǂ13
Issue: ǂIssue ǂ9
Chronology: 2012
DOI: 10.3390/ijms130912082
ID: 1032524