magistrsko delo
Amela Osmić (Author), Mateja Germ (Mentor)

Abstract

Selen (Se) in jod (I) sta esencialna elementa za ljudi in živali, medtem ko njuna esencialna vloga za rastline še ni dokazana. Obstaja tudi zelo malo podatkov o interakciji med Se in I pri rastlinah, zato je pomembno, da preučujemo hkraten vpliv obeh elementov na rastline, ki jih uporabljamo za prehrano ljudi. Približno 2/3 svetovne populacije ima zdravstvene težave, ki so povezane z nezadostnim vnosom Se in I s hrano v telo. Ena izmed najpreprostejših rešitev za to težavo je biofortifikacija oz. obogatitev poljščin s Se in I, katere namen je povečanje prenosa Se in I v prehransko verigo. V naši raziskavi so poskusne rastline predstavljale kalice kolerabice (Brassica oleracea L. var. gongylodes L.). Kolerabica je kapusnica, ki spada v družino križnic in je videti kot mešanec med zeljem in repo. Raziskavo smo izvajali na Oddelku za biologijo Biotehniške fakultete, na Katedri za ekologijo in varstvo okolja. Z raziskavo smo želeli ugotoviti, ali različne oblike Se in I posamezno ali v kombinaciji vplivajo na izbrane biokemijske in morfološke lastnosti pri kalicah kolerabice. Za dosego tega cilja smo si zastavili dve raziskovalni vprašanji. S prvim raziskovalnim vprašanjem smo poskušali ugotoviti, kako različne kemijske oblike Se in I ter njune kombinacije vplivajo na izbrane biokemijske in morfološke lastnosti kalic kolerabice, z drugim raziskovalnim vprašanjem pa smo poskušali ugotoviti, kako različne kemijske oblike Se in I ter njune kombinacije vplivajo na rast kalic kolerabice. Raziskava je potekala na slučajnostnem vzorcu, ki so ga predstavljala semena kolerabice. Poskus je bil sestavljen iz devetih obravnavanj. Vsako obravnavanje je obsegalo 4 ponovitve, v eni ponovitvi je vzorec predstavljalo 300 semen kolerabice. Kalice smo vzgojili iz semen, ki smo jih namočili v osem različnih raztopin z različnimi kombinacijami in oblikami Se in I ter v kontrolno raztopino, ki jo je predstavljala destilirana voda (dH2O). Ostale raztopine so poleg dH2O vsebovale posamezno dodan selenit (SeO32-) oz. selenat (SeO42-) s koncentracijo 10 mg Se/L, jodid (I-) oz. jodat (IO3-) s koncentracijo 1.000 mg I/L in kombinacije različnih oblik Se in I (SeO32-+ I-, SeO32-+ IO3-, SeO42- + I-, SeO42- + IO3-). Selen je bil v dH2O dodan v obliki natrijevega selenita (Na2SeO3) oz. natrijevega selenata (Na2SeO4), I pa v obliki kalijevega jodida (KI) oz. kalijevega jodata (KIO3). Merili smo koncentracijo klorofila a in b, karotenoidov, antocianov, UV-A absorbirajočih snovi, UV-B absorbirajočih snovi ter potencialno fotokemično učinkovitost fotosistema II (FS II). Na koncu poskusa smo izmerili še višino in maso kalic. Zaradi primerljivosti rezultatov smo celoten poskus po enakem postopku izvedli dvakrat.

Keywords

biologija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL PEF - Faculty of Education
Publisher: [A. Osmić]
UDC: 58(043.2)
COBISS: 11560265 Link will open in a new window
Views: 565
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Other data

Secondary language: English
Secondary title: The effect of selenium and iodine on selected biochemical and morphological characteristics in kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.)
Secondary abstract: Selenium (Se) and iodine (I) are essential elements for humans and animals, while their essential role for plants has not yet been established. There is also very little information about the interaction between selenium and iodine in plants, therefore it is important to study the combined effect of these two elements on plants that are used for human consumption. Approximately 2/3 of the world's population has health problems associated with insufficient intake of Se and I with diet. One of the easiest way to combat this problem is biofortification or enrichment of crops with Se and I, to increase the transfer of Se and I into the food chain. In our research we used kohlrabi sprouts (Brassica oleracea L. var. gongylodes L.). Kohlrabi is a brassica, which belongs to the family of Brassicaceae, and it looks like a hybrid between cabbage and turnip. The research was conducted at the Department of Biology at the Biotechnical Faculty in the Chain of Ecology and Environmental Protection. The aim of our research was to determine the effect of different forms of Se, I and their combinations on selected biochemical and morphological characteristics of the kohlrabi sprouts. To achieve this goal we have set ourselves two research questions. With the first research question we tried to examine how the different chemical forms of Se and I and combinations of both elements affect selected biochemical and morphological properties of kohlrabi sprouts. With the second research question we tried to examine how the different chemical forms of Se and I and combinations of both elements affect growth of kohlrabi sprouts. The study was conducted on a random sample, which were kohlrabi seeds. The experiment consisted of nine treatments. There were four replicates for each treatment with 300 seeds per one replicate. Sprouts were grown from seeds, which were soaked in eight different solutions with different combinations and forms of Se and I, and in the control solution which was distilled water (dH2O). Other solutions contained selenite (SeO32-) or selenate (SeO42-) with a concentration of 10 mg Se/L, iodide (I-) or iodate (IO3-) with a concentration of 1000 mg I/L, and their combinations (SeO32-+ I-, SeO32-+ IO3-, SeO42- + I-, SeO42- + IO3-). Selenium was applied to distilled water in the form of sodium selenite (Na2SeO3) and sodium selenate (Na2SeO4), respectively. Iodine was applied to distilled water in the form of potassium iodide (KI) and potassium iodate (KIO3), respectively. We measured the content of chlorophyll a and b, carotenoids, anthocyanins, UV-A and UV-B absorbing substances. We also measured potential photochemical efficiency of photosystem (PS II). At the end of the experiment we measured the weight and length of the sprouts. In order to compare the results the entire experiment was carried out twice.
Secondary keywords: botany;botanika;
File type: application/pdf
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Ljubljani, Pedagoška fak., Predmetno poučevanje, Biologija in gospodinjstvo
Pages: X, 43 str.
ID: 10838464