Effect of base sequence on G-wire formation in solution

Lea Spindler (Author), Martin Rigler (Author), Irena Drevenšek Olenik (Author), Nason Ma'ani Hessari (Author), Mateus Webba da Silva (Author)

Abstract

The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating.

Keywords

DNA;dynamic light scattering;DLS;polyacrylamide gel electrophoresis;PAGE;genetics;

Data

Language:	English
Year of publishing:	2010
Typology:	1.01 - Original Scientific Article
Organization:	UM FS - Faculty of Mechanical Engineering
UDC:	53
COBISS:	23737127
ISSN:	2090-021X
Parent publication:	G-Quadruplex Nucleic Acids
Views:	966
Downloads:	328
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	DNK;dinamično sipanje svetlobe;DLS;poliakrilamidna gelska elektroforeza;PAGE;genetika;
URN:	URN:SI:UM:
Type (COBISS):	Scientific work
Pages:	str. 431651-1-431651-8
Volume:	ǂVol. ǂ2010
Chronology:	2010
DOI:	10.4061/2010/431651
ID:	10842665