Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration

Jakob Naranđa (Author), Maja Sušec (Author), Uroš Maver (Author), Lidija Gradišnik (Author), Mario Gorenjak (Author), Andreja Vukasović (Author), Alan Ivković (Author), Marjan Rupnik (Author), Matjaž Vogrin (Author), Peter Krajnc (Author)

Abstract

Development of artificial materials for the facilitation of cartilage regeneration remains an important challenge in orthopedic practice. Our study investigates the potential for neocartilage formation within a synthetic polyester scaffold based on the polymerization of high internal phase emulsions. The fabrication of polyHIPE polymer (PHP) was specifically tailored to produce a highly porous (85%) structure with the primary pore size in the range of 50–170 μm for cartilage tissue engineering. The resulting PHP scaffold was proven biocompatible with human articular chondrocytes and viable cells were observed within the materials as evaluated using the Live/Dead assay and histological analysis. Chondrocytes with round nuclei were organized into multicellular layers on the PHP surface and were observed to grow approximately 300 μm into the scaffold interior. The accumulation of collagen type 2 was detected using immunohistochemistry and chondrogenic specific genes were expressed with favorable collagen type 2 to 1 ratio. In addition, PHP samples are biodegradable and their baseline mechanical properties are similar to those of native cartilage, which enhance chondrocyte cell growth and proliferation.

Keywords

poliestri;polyHIPE;polimerizacija;polyester;polymerization;

Data

Language:	English
Year of publishing:	2016
Typology:	1.01 - Original Scientific Article
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC:	66
COBISS:	19662102
ISSN:	2045-2322
Views:	1241
Downloads:	358
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	poliestri;polyHIPE;polimerizacija;
URN:	URN:SI:UM:
Type (COBISS):	Scientific work
Pages:	str. 1-11
Volume:	ǂVol. ǂ6
Issue:	ǂart. no. ǂ28695
Chronology:	Published online: 24 June 2016
DOI:	10.1038/srep28695
ID:	10844204