Janja Trček (Author), Luka Lipoglavšek (Author), Gorazd Avguštin (Author)

Abstract

Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has oft en been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production.

Keywords

mikrobiologija;ocetnokislinske bakterije;pretočna citometrija;microbiology;acetic acid bacteria;flow cytometry;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FNM - Faculty of Natural Sciences and Mathematics
UDC: 579.66
COBISS: 22612232 Link will open in a new window
ISSN: 1330-9862
Views: 27226
Downloads: 325
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: mikrobiologija;ocetnokislinske bakterije;pretočna citometrija;
URN: URN:SI:UM:
Type (COBISS): Scientific work
Pages: str. 108-112
Volume: ǂVol. ǂ54
Issue: ǂno. ǂ1
Chronology: 2016
DOI: 10.17113/ftb.54.01.16.4041
ID: 10850643