Pridobivanje transglutaminaze iz bakterije Streptomyces platensis

magistrsko delo

Vida Lang (Author), Maja Leitgeb (Mentor), Maja Čolnik (Co-mentor)

Abstract

Streptomyces platensis je bakterija iz rodu Streptomyces. V rod Streptomyces spadajo nitaste bakterije, ki jih najdemo v prsti. Bakterije Streptomyces uspevajo pri pH 6,5 % 8,0 in v temperaturnem območju med 25 °C in 35 °C. Za S. platensis je značilna proizvodnja zunajceličnih encimov, ki se uporabljajo za industrijske namene. Namen magistrske naloge je bil ugotoviti kako sprememba pH v celični suspenziji S. platensis vpliva na maso celic S. platensis, aktivnost encima transglutaminaza in koncentracijo skupnih proteinov v bakterijski suspenziji S. platensis. Celično suspenzijo S. platensis smo inkubirali v inkubacijskem stresalniku 8 dni pri temperaturi 28 °C in ob rahlem stalnem stresanju. Naredili smo 3 izvedbe, pri katerih smo v procesu inkubiranja celične suspenzije S. platensis spreminjali pogoje pH. Pri vseh 3 izvedbah inkubiranja celične suspenzije S. platensis v odvisnosti od inkubacijskega časa smo ugotovili, da medtem ko se je pH celične suspenzije zniževal, so koncentracija proteinov, aktivnost transglutaminaze in prirast suhe mase celic naraščale. Po treh različnih izvedbah se je izkazalo, da je za gojenje celične suspenzije S. platensis najugodnejši začetni pH 6,7 in inkubiranje pri temperaturi 28 °C ob rahlem stresanju ter brez dodatnega spreminjanja pH. Spreminjanje oziroma uravnavanje pH na začetku procesa ali med celotnim procesom inkubiranja celične suspenzije S. platensis ni ugodno za uspešno gojenje bakterije S. platensis, saj se celoten proces pospeši in bakterije hitreje propadejo.

Keywords

magistrska dela;S. platensis;aktivnost transglutaminaze;koncentracija proteinov;pH;masa celic;

Data

Language:	Slovenian
Year of publishing:	2017
Typology:	2.09 - Master's Thesis
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	[V. Lang]
UDC:	543.645.4:579.873.7(497.411)(043.2)
COBISS:	23361288
Views:	1609
Downloads:	198
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Extraction of transglutaminase from bacterium Streptomyces platensis
Secondary abstract:	Streptomyces platensis is bacteria from the genus of Streptomyces. Part of the genus Streptomyces are thread-like bacteria, which are found in the soil. Bacteria Streptomyces thrive at pH 6,5 % 8,0 and in the temperature range between 25 and 35 °C. S. platensis are known for the production of extracellular enzymes, which are being used for industrial purposes. The purpose of this master thesis was to find out how changes of pH in the cellular suspension of S. platensis affect the mass of cells of S. platensis, how they affect the activity of transglutaminase enzyme and how they affect the concentration of total proteins in bacterial suspension. We have incubated cellular suspension of S. platensis in an incubator shaker for 8 days at a temperature of 28 °C and constant mild shaking. We have performed three trials, during which we have been changing pH conditions in the process of incubation of cellular suspension of S. platensis. At all three trials of incubation of the cellular suspension of S. platensis we have discovered in respect to the incubating time that while the pH of the cellular suspension was dropping, the concentration of proteins, the activity of transglutaminase and the growth of the dry mass of cells were rising. After three different trials it has turned out, that for the growth of the cellular suspension of S. platensis it is ideal to have a starting value of pH to be 6,7 and for incubating to occur at 28 °C with constant mild shaking and without additional altering of the pH value. Changing the value of pH either at the start or during the process of incubation of the cellular suspension of S. platensis is not beneficial for the successful growth of the bacteria S. platensis as the entire process is being accelerated and bacteria decay faster.
Secondary keywords:	master theses;S. platensis;activity of transglutaminase;concentration of proteins;pH;cellular mass;
URN:	URN:SI:UM:
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo
Pages:	IX f., 64 str.
ID:	10852661