Vpliv različnih aditivov v izbranih polimernih materialih na rast gram pozitivnih in gram negativnih mikroorganizmov

diplomsko delo univerzitetnega študijskega programa I. stopnje

Nika Kučuk (Author), Mateja Primožič (Mentor), Maja Leitgeb (Co-mentor)

Abstract

Glavni namen diplomske naloge je bil določiti tisti polimerni material, ki bo najbolj zaviral rast mikroorganizmov. Antimikrobno aktivnost izbranih polimernih materialov z dodatkom različnih koncentracij cinkovega oksida (ZnO) ter drugih aditivov (stearinska kislina) smo testirali na gram negativni bakteriji Escherichia coli in gram pozitivni bakteriji Staphylococcus aureus. Analizni postopek smo izvedli v skladu z ISO standardom 22196:2007, kjer je opisana metoda, s katero smo določili antimikrobne lastnosti izbranih polimernih materialov. Uporabili smo osem različnih materialov, od katerih so bili štirje iz polietilena (PE), ostali štirje pa iz polipropilena (PP). Izbrani polimerni materiali so vsebovali različne koncentracije ZnO ter preostalih primesi. Antimikrobno aktivnost materialov smo določevali na osnovi primerjave med številom preživelih bakterijskih kolonij na testnih vzorcih, ki so vsebovali ZnO, s številom preživelih bakterijskih kolonij na slepih vzorcih, ki niso vsebovali ZnO ali drugih primesi. Rezultati so pokazali, da ZnO in dodane primesi v vseh uporabljenih polimernih materialih uspešno inhibirajo rast in razmnoževanje gram pozitivne bakterije S. aureus. Popolna inhibicija na gram negativno bakterijo E. coli pa je bila dokazana le pri dveh izbranih polimerih. Metodo po ISO 22196:2007 smo potrdili na dveh komercialnih materialih, ki sta obdelana s tehnologijo Microban® in GermoSAFE ter zagotavljata antimikrobno učinkovitost.

Keywords

antimikrobne lastnosti;inhibicija rasti mikroorganizmov;cinkov oksid;polimerni materiali;polietilen;polipropilen;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2017
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[N. Kučuk]
UDC:	665.652.2(043.2)
COBISS:	21049366
Views:	978
Downloads:	46
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	The effect of different additives in the selected polymeric materials on the growth of gram positive and gram negative microorganisms
Secondary abstract:	The main purpose of this diploma thesis was to determine polymeric material that will most inhibit the growth of microorganisms. The antimicrobial activity of the selected materials with the addition of different concentrations of zinc oxide (ZnO) and other additives (stearic acid) on gram negative bacteria Escherichia coli and gram positive bacteria Staphylococcus aureus was tested. The analytical procedure was carried out in accordance with ISO standard 22196:2007, which describes a method for determining the antimicrobial properties on selected polymeric materials. Eight different materials, of which four were made of polyethylene (PE) and the other four of polypropylene (PP) were used. The selected polymeric materials contained different concentrations of ZnO and other additives. The antimicrobial activity of the materials on the basis of comparison between the number of viable bacteria colonies recovered from samples treated with ZnO particles, with the number of viable bacteria colonies recovered from zero samples that did not contain ZnO or other additives was determined. The results showed that ZnO and added additives in all used polymeric materials successfully inhibit the growth and reproduction of gram positive bacteria S. aureus. Complete inhibition of gram negative bacteria E. coli was proved only on two selected polymers. The ISO 22196:2007 method was confirmed on two commercial materials which provide antimicrobial efficacy, treated with technology Microban® and GermoSAFE.
Secondary keywords:	antimicrobial properties;inhibition of the growth of microorganisms;zinc oxide;polymeric materials;polyethylene;polypropylene;
URN:	URN:SI:UM:
Type (COBISS):	Bachelor thesis/paper
Embargo end date (OpenAIRE):	2020-09-14
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	VIII, 39 str.
ID:	10859442