Milena Ivanović (Author), Maša Islamčević Razboršek (Author), Mitja Kolar (Author)

Abstract

Several phenolic acids (PAs), caffeic, vanillic, syringic, p-coumaric and ferulic acid, found in Slovenian red wines were studied using gas chromatography and mass spectrometry (GC-MS). For isolation of the PAs from wine samples, solid phase extraction (SPE) using hydrophilic modified styrene - HLB cartridges was used. The bound PAs were extracted after basic hydrolysis and o-coumaric acid was used as the internal standard (ISTD). The method developed was validated and the linear concentration range for all analytes was from 1 to 100 mg L$^{-1}$ with correlation coefficients above 0.999. We show that the method is repeatable (RSD<2%), recoveries were above 96%, and LOD and LOQ values were acceptable. In all of the wine samples tested, caffeic and p-coumaric acid were determined to be the predominant PAs (17-72 mg L$^{-1}$), while other compounds were found in lower concentrations. Principal Component Analysis (PCA) and Cluster Analysis (CLU) were used to study differences between wines related towards varieties and Slovenian wine regions. The results demonstrate that variety has more influence on PAs content than wine regions in Slovenian red wines.

Keywords

phenolic acids;Slovenian red wines;gas chromatography;mass spectrometry;PCA;CLU;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
UDC: 66
COBISS: 19728662 Link will open in a new window
ISSN: 1318-0207
Views: 1566
Downloads: 340
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Other data

Secondary language: Slovenian
Secondary abstract: Izbrane fenolne kisline: kavno, vanilinsko, siringinsko, kumarinsko in ferulno smo v različnih rdečih slovenskih vinih analizirali z uporabo GC-MS. Za izolacijo smo uporabljali ekstrakcijski postopek na HLB SPE kolonicah, ki vsebuje hidrofilno modificiran stiren. Pri delu smo vezane fenolne kisline hidrolizirali, kot interni standard pa smo uporabljali orto-kumarinsko kislino. Razvito metodo smo validirali: linearno koncentracijsko območje fenolnih kislin je med 1 in 100 mg L$^{-1}$, korelacijski koeficienti so bili nad 0,999. Potrdili smo dobro ponovljivost, RSD je znašal pod 2%, izkoristke nad 96% in sprejemljive vrednosti LOD ter LOQ. Ugotovili smo, da sta v vzorcih slovenskih vin prevladujoči fenolni spojini kavna in para-kumarna kislina (17–72 mg L$^{-1}$), medtem ko ostale spojine najdemo v nižjem koncentracijskem območju. Metodo glavnih osi (PCA) in analizo klastrov (CLU) smo uporabili za študij podobnosti in razlik med vzorci glede vsebnosti in deležev fenolnih kislin. Potrdili smo korelacijo in večji vpliv sorte grozdja kot vinorodne dežele oziroma regije, iz katere vzorci vin izvirajo.
Secondary keywords: fenolni acidi;slovenska rdeča vina;plinska kromatografija;masna spektrometrija;
URN: URN:NBN:SI
Type (COBISS): Scientific work
Pages: str. 661-669
Volume: ǂVol. ǂ63
Issue: ǂno. ǂ3
Chronology: 2016
DOI: 10.17344/acsi.2016.2534
ID: 10860275