In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants

Abstract

The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.

Keywords

mikropropagacija;tkivne kulture;hortenzija;Hydrangea macrophylla;sterilizacija rastlin;

Data

Language:	Slovenian
Year of publishing:	2016
Typology:	1.01 - Original Scientific Article
Organization:	UM FKBV - Faculty of Agriculture
UDC:	631.526:582.785
COBISS:	4309804
ISSN:	1580-8432
Parent publication:	Agricultura
Views:	1204
Downloads:	295
Average score:	0 (0 votes)
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Other data

Secondary language:	English
Secondary title:	Iniciacija rastlinske tkivne kulture lončne in vrtne hortenzije (Hydrangea macrophylla) v in vitro razmerah
Secondary abstract:	Proučevali smo vpliv različnih načinov sterilizacije na uspešno vzpostavitev tkivne kulture treh genotipov hortenzije (Hydrangea macrophylla). Ugotovili smo, da obstajajo razlike med različnimi načini sterilizacije. Sterilizacija z dikloroizocianurno kislino v kombinaciji s srebrovim nitratom je bila uspešnejša od sterilizacije z uporabo NaOCl. Na uspešnost sterilizacije je vplival tudi genotip. Lončne hortenzije (genotipa G2 in G3) so kazale večjo sposobnost sterilizacije (pri genotipu G2 smo tako dobili 45.00 %, pri G3 pa 55.00 % uspešno steriliziranih poganjkov) v primerjavi z vrtno hortenzijo, kjer je bilo največ okuženih poganjkov (58.75 %). Za namnoževanje poganjkov se je kot najboljše izkazalo gojišče McCown z dodatkom 2 mg/L BAP in 0.005 NAA, na katerem je bil faktor namnoževanja 1,31 v petih tednih. Najbolje je koreninil genotip 2 na McCown osnovnem gojišču z dodano 0,5 mg/L IBA in sicer je na tem gojišču koreninilo 100 % poganjkov.
Secondary keywords:	micropropagation;tissue culture;hydrangea;Hydrangea macrophylla;plant disinfection;
URN:	URN:NBN:SI
Type (COBISS):	Scientific work
Pages:	str. 65-69
Volume:	ǂVol. ǂ13
Issue:	ǂNo. ǂ1/2
Chronology:	Dec. 2016
DOI:	10.1515/agicultura-2017-0008
ID:	10878849