diplomsko delo
Jana Sitar (Author), Martina Bergant Marušič (Mentor)

Abstract

Humani virusi papiloma so eden najpogostejših vzrokov spolno prenosljivih bolezni in nastanka nekaterih vrst raka, kot so rak materničnega vratu, rakava obolenja na spolovilih ter rak glave in vratu. V diplomskem delu smo ugotavljali vpliv notranjih in zunanjih dejavnikov na nastanek in infektivnost virusov HPV-16, določali pa smo tudi strukturne lastnosti virusnega plašča. Kot notranji dejavnik smo uporabili gostiteljski protein SNX17, ki je ključen za okužbo HPV, kot zunanji dejavnik pa nanodelce titanovega dioksida, ki so pogosta sestavina kozmetičnih izdelkov. Pri pridobivanju virusnih delcev HPV-16 v celični liniji 293TT smo ugotovili, da protein SNX17 in nanodelci TiO2 ne vplivajo na količino virusnih delcev, vplivajo pa na njihovo infektivnost. Potrdili smo, da je protein SNX17 ključnega pomena za okužbo HPV, saj so imeli mutirani virusni delci AA2 bistveno zmanjšano stopnjo okužbe. Podobno smo ugotovili pri uporabi nanodelcev TiO2, kjer je bil upad infektivnosti sorazmeren koncentraciji uporabljenih nanodelcev med fazo nastanka virusnih delcev. Pri strukturnih lastnostih virusnega plašča smo analizirali količino in vrsto vključene reporterske DNA, stabilnost plašča in razmerje proteinov L1 : L2 v plašču. Ugotovili smo, da vezava plaščnega proteina L2 s SNX17 in prisotnost nanodelcev TiO2 na te lastnosti ne vplivata. Zaključili smo, da izbrani okoljski dejavniki, protein SNX17 in nanodelci TiO2 ne vplivajo na osnovne značilnosti nastanka virusnih delcev HPV-16, vplivajo pa na njihovo učinkovitost okužbe gostiteljskih celic.

Keywords

HPV;plaščni proteini;okužbe;TiO2;SNX17;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UNG FZO - Faculty of Environmental Sciences
Publisher: [J. Sitar]
UDC: 577
COBISS: 4957435 Link will open in a new window
Views: 5440
Downloads: 234
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Other data

Secondary language: English
Secondary title: The impact of internal and external environmental factors on the formation of HPV viruses
Secondary abstract: Human papillomaviruses are one of the most common causes of sexually transmited diseases and development of certain types of cancer, such as cervical cancer, cancer on genial areas and neck and head cancer. In this paper we evaluated influence of internal and external factors on production and infectivity of the HPV16, we also determined structual properties of viral capsids. As an internal factor, we used SNX17 host protein, which is essential for HPV infection, and as an external factor, we used TiO2 nanoparticles, that are common ingredient in cosmetic products. In the production of HPV16 viral particles in 293TT cell line, we found that SNX17 protein and TiO2 nanoparticles have no effect on the amount of viral particles, but they do affect their infectivity. We confirmed that the SNX17 protein is crucial for HPV infection, as the mutant viral particles AA2 had a significant reduced infection rate. Similar effect was also observed in the use of TiO2 nanoparticles, where the decline of infectivity was proportional to the concentration of nanoparticles used during the production phase. In the structural properties of the viral capsid, we analized the amount and type of the reporter DNA, the capsid stability and the L1:L2 protein ratio in the capsid. We have found that binding of the L2 capsid protein with SNX17 and presence of TiO2 nanoparticles, do not affect these properties. We concluded that the selected environmental factors, SNX17 protein and TiO2 nanoparticles, do not affect the basic characteristics of the viral partices of the HPV16, but affect their infection efficiency in host cells.
Secondary keywords: HPV;capsid proteins;infection;TiO2;SNX17;
URN: URN:SI:UNG
Type (COBISS): Bachelor thesis/paper
Thesis comment: Univ. v Novi Gorici, Fak. za znanosti o okolju
Pages: X, 31, II str.
ID: 10879775