Sabina Horvat (Author), Aleksander Mahnič (Author), Martin Breskvar (Author), Sašo Džeroski (Author), Maja Rupnik (Author)

Abstract

Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota. A marked decrease of microbial diversity was observed in conditioned medium of both tested ribotypes. The majority of differences occurred within the phylum Firmicutes, with a general decrease of gut commensals with putative protective functions (i.e. Lactobacillus, Clostridium_XIVa) and an increase in opportunistic pathogens (i.e. Enterococcus). Bacterial populations in conditioned medium differed between the two C. difficile ribotypes, 027 and 014/020 and are likely associated with nutrient availability. Fecal microbiota cultivated in medium conditioned by E. coli, Salmonella Enteritidis or Staphylococcus epidermidis grouped together and was clearly different from microbiota cultivated in C. difficile conditioned medium suggesting that C. difficile effects are specific. Our results show that the changes observed in microbiota of CDI patients are partially directly influenced by C. difficile.

Keywords

Clostridium difficile;infection;gut microbiota;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM MF - Faculty of Medicine
UDC: 579.852.13
COBISS: 512751416 Link will open in a new window
ISSN: 2045-2322
Views: 1016
Downloads: 338
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Other data

Secondary language: Slovenian
Secondary keywords: Clostridium difficile;
URN: URN:SI:UM:
Type (COBISS): Scientific work
Pages: 11 str.
Issue: ǂVol. ǂ7
Chronology: 2017
DOI: 10.1038/s41598-017-15434-1
ID: 10888629