magistrsko delo
Alenka Žnidarčič (Author), Marko Kreft (Reviewer), Primož Rožman (Mentor), Primož Rožman (Thesis defence commission member), Mojca Narat (Thesis defence commission member), Marko Kreft (Thesis defence commission member)

Abstract

Mezenhimske stromalne celice (MSC) so eden glavnih virov za celične terapije in tkivno inženirstvo. Sposobne so samoobnavljanja in diferenciacije, lahko uravnavajo imunski odziv in sodelujejo pri celjenju poškodb. S staranjem se njihove regenerativne sposobnosti zmanjšajo. Pri terapijah z MSC se zato poraja vprašanje, kako starost darovalca in celic vpliva na izid zdravljenja. V magistrskem delu smo skušali ugotoviti, kako starost miši BALB/c vpliva na lastnosti njihovih MSC. S pretočno citometrijo smo spremljali izražanje celičnih označevalcev med gojenjem mladih in starih celic, spremljali smo proliferacijo celic s testom MTT, določali smo sposobnost migracije celic s testom celjenja ran in prisotnost senescentnih celic v kulturi. Ugotovili smo, da se mlade celice hitreje delijo, imajo nižji delež senescentnih celic v kulturi in slabše migracijske sposobnosti v primerjavi s starimi celicami. Med izražanjem celičnih označevalcev med skupinama nismo opazili razlik.

Keywords

mezenhimske stromalne celice;miši;kostni mozeg;staranje;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Žnidarčič]
UDC: 602.9:591.81:576.5(043.2)
COBISS: 8908153 Link will open in a new window
Views: 1083
Downloads: 569
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: English
Secondary title: Characterization of mesenchymal stromal cells, isolated from bone marrow of young and old BALB/c mice
Secondary abstract: Mesenchymal stromal cells are one of the main sources for cell therapy and tissue engineering. They are capable of self-renewal and differentiation. They can regulate a body's immune response and participate in its wound-healing process. Their regenerative abilities reduce as they age. MSC therapies therefore raise a question of how the age of the donor affects the outcome of the treatment. In this master's thesis, we tried to determine how the age of BALB/c mice affects the properties of their MSC. We monitored the expression of cell markers during cell culture with flow cytometry. We monitored cell proliferation rate with the MTT assay and analyzed mouse MSC's migration capacities with wound-healing assay. By measuring the β-galactosidase activity we determined the presence of senescent cells in culture. We discovered that young cells divide more rapidly, have a lower proportion of senescent cells in culture and have lower migration capacity when compared to older cells. The expression of cell markers is the same for both cell groups.
Secondary keywords: mesenchymal stromal cells;mice;bone marrow;ageing;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: XI, 58 f.
ID: 10915430