magistrsko delo
Gabrijela Petek (Author), Lidija Fras Zemljič (Mentor), Ivan Anžel (Co-mentor), Zdenka Peršin (Co-mentor)

Abstract

Trend razvoja medicinskih tekstilnih materialov za zdravljenje in celjenje ran, temelji na uporabi materialov, ki so pripravljeni na osnovi naravnih učinkovin z bioaktivnimi lastnostmi. Tradicionalno oskrbo ran je zamenjala uporaba sodobnih oblog, med katerimi je v zadnjem času zlasti privlačna priprava nanovlaknastih materialov. V magistrski nalogi smo se osredotočili na pripravo naprednih medicinskih tekstilij na osnovi hitozana, ki smo jih izdelali s postopkom elektropredenja. Tako izdelane tekstilije so zaradi velike gostote por, velikega razmerja med specifično površino in volumnom, visoke prepustnosti ter majhnega premera vlaken, primerna za sproščanje učinkovin. V našem primeru smo uporabili učinkovini katehin in resveratrol, ki s svojim antioksidativnim značajem ugodno vplivata na celjenje ran. Tekoče formulacije smo pripravili po postopku ionotropnega geliranja, ter tako izbrano učinkovino ujeli v hitozanske nanodelce. Karakterizacija pripravljenih disperzij hitozanskih nanodelcev z ujetimi polifenoli je potekala na podlagi določevanja velikosti delcev (DLS) ter vrednotenja antioksidativne in protimikrobne učinkovitosti. Iz pripravljenih tekočih formulacij smo s postopkom elektropredenja pripravili kompozitne vzorce medicinskih tekstilij, pri čemer je bilo potrebno postopek elektropredenja optimizirati s strani predilne raztopine ter parametrov predenja. Z uporabo Fourier transformirane infrardeče spektroskopije (FTIR), vrstične elektronske spektroskopije (SEM) in rentgenske fotoelektronske spektroskopije (XPS) smo pripravljene materiale okarakterizirali z fizikalno-kemijskega vidika ter ovrednotili učinek antioksidativnosti in protimikrobnosti. Na koncu smo izvedli analizo in vitro sproščanja učinkovin. Dokazali smo, da so tako pripravljeni kompozitni materiali multifunkcionalni z vidika bioaktivnosti, saj izkazujejo protimikrobne in antioksidativne lastnosti. Hkrati omogočajo tudi kontrolirano sproščanje učinkovin, s čimer izkazujejo potencial za uporabo v medicinskih aplikacijah, predvsem na področju zdravljenja in celjenja ran.

Keywords

medicinske tekstilije;hitozan;katehin;resveratrol;elektropredenje;nanovlakna;DLS;FTIR;SEM;XPS;antioksidativnost;protimikrobno testiranje;in vitro sproščanje;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FS - Faculty of Mechanical Engineering
Publisher: [G. Petek]
UDC: 677.017.86:615.4(043.2)
COBISS: 22145814 Link will open in a new window
Views: 848
Downloads: 131
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Other data

Secondary language: English
Secondary title: Development of medical textiles using polyphenols and polysaccharides
Secondary abstract: Development trend of medical textiles for the wound treatment and wound healing represents the use of the materials, which are prepared from natural substances with bioactive properties. Traditional wound care has been replaced by the use of modern wound dressings among which are recently especially attractive nanofiberous materials. In this master’s work, we focused on the preparation of the advanced medical textiles, made from chitosan via electrospinning process. So prepared materials are due to high porosity of structure, high specific surface area to volume ratio, a high permeability and small fiber diameter, suitable for the release of the substances. We used catechin and resveratrol because of their antioxidative character, which favorably affect to the wound healing process. Liquid formulation were prepared by ionoic gelation method according to which catechin and resveratrol were encapsulated in chitosan nanoparticles. Nanoparticles based formulations were characterized by determination of the particle size (DLS) and evaluation of antioxidant and antimicrobial efficiency. Composite medical textiles samples were prepared by electrospinning process from the prepared liquid formulation. From the standpoint of used spinning solution and spinning parameter was process optimized. With Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were prepared samples characterized. We also evaluated antioxidative and antimicrobial properties of functionalized materials. At the end, we performed the analysis of the in vitro release. It was proven that prepared materials are bioactive, because of their antimicrobial and antioxidant properties. At the same time, prepared medical textiles also enable release of the substances. They show potential for use in medical applications, in particular for the wound treatment and wound healing.
Secondary keywords: medical textiles;chitosan;catechin;electrospinning;nanofibers;antioxidativity;antimicrobial testing;in vitro release;
URN: URN:SI:UM:
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za strojništvo, Tehniško varstvo okolja
Pages: XII, 84 str.
ID: 10936734