magistrsko delo
Ylenia Prodan (Author), Polona Zalar (Reviewer), Rok Kostanjšek (Mentor), Peter Trontelj (Co-mentor)

Abstract

Kljub temu, da je človeška ribica najbolj prepoznavna žival dinarskega podzemlja, sta njena biologija in ekologija zaradi težke dostopnosti njenega naravnega okolja še vedno slabo raziskani. Zaradi prepoznanega pomena kožne mikrobiote za zdravje in dobrobit vretenčarskih gostiteljev, smo se v nalogi odločili podrobneje raziskati kožni bakteriom človeške ribice. Profil bakterijske združbe na koži človeške ribice smo raziskali z metagenomsko analizo sedmih vzorcev kože in enega vzorca vode. Prevladujoči taksoni, najdeni v vseh vzorcih kože, so bili: Sphingomonadaceae, Bradyrhizobiaceae, Microbacterium, Burkholderiaceae in Flavobacteriaceae. Ti po vsej verjetnosti izvirajo iz vodnega okolja in najverjetneje predstavljajo normalno mikrobioto kože človeške ribice. Primerjava bakterijske združbe kože istega osebka v dveh zaporednih letih kaže tako na ohranjenost prevladujočih taksonov, kot tudi na časovno dinamiko manjšega dela kožnega bakterioma človeške ribice. Med vzorci izrazito odstopa vzorec s kože črne človeške ribice. Ta je izjemno obremenjen z enterobakterijami in aeromonadami, ki skoraj povsem izrivajo taksone normalne kožne mikrobiote. Normalna kožna mikrobiota človške ribice doslej ni bila poznana, njena prisotnost pa ima velik potencial pri ohranjanju te ogrožene vrste in zagotavljanju ustreznih pogojev pri njenem gojenju v umetnih in seminaravnih pogojih.

Keywords

človeška ribica;Proteus anguinus;kožni mikrobiom;mikrobne združbe;16S rRNA;sekvenčne reakcije naslednje generacije;metagenomska analiza;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Y. Prodan]
UDC: 579.25/.26:597.92:577.2.088
COBISS: 4910968 Link will open in a new window
Views: 1557
Downloads: 386
Average score: 0 (0 votes)
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Other data

Secondary language: English
Secondary title: Skin microbiota of blind cave salamander (Proteus anguinus)
Secondary abstract: The most recognizable animal of the Dinaric underworld is certainly the blind cave salamander or proteus. However, it's biology and ecology are still understudied, mainly due to the inaccessibility of its natural environment. Regarding well recognized importance of the skin microbiota for the health and well-being of the host in other vertebrates, we decided to explore the skin microorganisms of the proteus. The profile of microbial groups on the skin was determined with metagenomic analysis of skin and compared with the water sample. The prevailing taxa found in all skin samples were: Sphingomonadaceae, Bradyrhizobiaceae, Microbacterium, Burkholderiaceae and Flavobacteriaceae. These originate from surrounding water and most likely represent the normal skin microbiota of the proteus. Comparison of skin bacteriomes of the same individual in two consecutive years showed both the preservation of the dominant taxa and the temporal dynamics of a small part of the skin bacteria. Significant shift in skin bacteriome composition was observed in the sample of black subspecies, in which normal bacteriome was almost completely replaced by enterobacteria and aeromonads. The observed presence of normal skin bacteriome in proteus has not been known by now. This knowledge has a significat potential in conservation of this endangered amphibian and in providing suitable conditions for keeping these animals in captivity for scientific, exhibiton or revitalization purposes.
Secondary keywords: blind cave salamander;Proteus anguinus;microbial communities;16S rRNA;next generation sequencing;metagenomic analysis;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: IX, 64 f.
ID: 10938688