Inhibitorne lastnosti borovnic na rast mikrobnih celic

diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje

Jerneja Babič (Author), Maja Leitgeb (Mentor), Mateja Primožič (Co-mentor), Maša Knez Hrnčič (Co-mentor)

Abstract

Namen diplomske naloge je proučiti antimikrobne lastnosti ameriških borovnic (Vaccinium corymbsum L.) ter navadnih borovnic (Vaccinium myrtillus L.) na rast različnih mikrobnih celic kot so Escherichia coli, Staphylococcus aureus, Bacillus cereus, Aspergillus brasiliensis, Candida albicans, Pseudomonas aeruginosa ter Pseudomonas fluorescens. Z ekstrakcijo po Soxhletu smo pripravili več različnih ekstraktov borovnic, kot so etanolni ekstrakt ameriške borovnice, etanolni ekstrakt liofilizirane ameriške borovnice ter ekstrakti iz navadne borovnice z etanolom, metanolom in acetonom. Poleg ekstraktov navadnih ter ameriških borovnic smo kot testne vzorce uporabili tudi svež sok ameriške borovnice ter liofiliziran sok ameriške borovnice. Inhibitorne lastnosti smo ugotavljali z disk difuzijsko metodo, tako da smo sterilne diske namestili na gojišča z mikroorganizmi ter na diske nanesli vzorce borovnic. Ugotovili smo, da borovnice ne zavirajo rast kvasovk kot sta Candida albicans ter Aspergillus brasiliensis. Največji inhibitorni učinek na rast vseh testnih bakterij je bilo zaznati v primeru metanolnega ekstrakta navadne borovnice, medtem ko je najslabše inhibitorne lastnosti bilo moč zaznati v primeru svežega soka ameriške borovnice ter liofiliziranega soka ameriške borovnice. Testirani vzorci ameriških in navadnih borovnic so najbolj inhibitorno delovali na rast bakterije Pseudomonas aeruginosa, ki je Gram negativna bakterija, saj je pri vseh testnih vzorcih bilo zazanati cono inhibicije resti mikroorganizma. Najslabše inhibitorne lastnosti testiranih vzorcev pa se kažejo pri Staphylococcus aureus, ki je Gram pozitivna bakterija.

Keywords

ameriške borovnice;navadne borovnice;antimikrobne lastnosti;inhibicija;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2018
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[J. Babič]
UDC:	582.688.31(043.2)
COBISS:	21606166
Views:	1000
Downloads:	197
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Inhibitory properties of blueberries on the growth of microbial cells
Secondary abstract:	The purpose of this work was to study the antimicrobial properties of blueberries (Vaccinium corymbsum L.) and bilberries (Vaccinium myrtillus L.) on the growth of various microbial cells such as Escherichia coli, Staphylococcus aureus, Bacillus cereus, Aspergillus brasiliensis, Candida albicans, Pseudomonas aeruginosa and Pseudomonas fluorescens. With Soxhlet extraction, we prepared several different test samples, such as blueberry ethanol extract, ethanol extract of lyophilized blueberry and ethanol, methanol and acetone bilberry extract. Beside the extracts of bilberry and blueberry, fresh juice of blueberry and lyophilized juice was used as test samples. The inhibitory properties were determined by the discs method. The sterile discs were placed on media with microorganisms and then samples of blueberries and bilberries were applied on the discs. The results show that test samples of blueberries and bilberries do not have inhibitory properties on yeast growth, such as Candida albicans and Aspergillus brasiliensis. Inhibition has appeared in all bacteria. The greatest inhibition was caused by the methanol extract of bilberries, the slightest inhibition was caused by the fresh blueberry juice and the lyophilized juice of blueberries. The highest inhibition was observed in Pseudomonas aeruginosa who is Gram negative bacteria and the smallest in Staphylococcus aureus who is Gram positive bacteria.
Secondary keywords:	blueberry;bilberry;antimicrobial properties;inhibition;
URN:	URN:SI:UM:
Type (COBISS):	Bachelor thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	X, 38 str.
ID:	10942513