Molekularna diagnostika limfomov

Ira Koković (Author), Srdjan Novaković (Author)

Abstract

Določanje klonalnosti limfoidnih proliferacij in dokazovanje specifičnih genetskih sprememb ima pomembno vlogo v diagnostiki limfoidnih neoplazem, saj omogoča ločevanje med neoplastičnimi lezijami in reaktivnimi procesi. Klonalno populacijo limfoidnih celic lahko dokažemo s pomnoževanjem preurejenih genov, ki kodirajo polipeptidne verige receptorjev na površini celic B in T. Z metodo PCR lahko dokažemo tudi kromosomske translokacije, značilne za posamezne vrste limfomov. Uvedli smo tehnike za določanje klonalnosti limfoidnih proliferacij in dokazovanje kromosomskih translokacij t(11;14) pri plaščnoceličnem ter t(14;18) pri folikularnem limfomu, ki temeljijo na polimerazni verižni reakciji. Tehnike so preproste, hitre in uporabne za molekularnobiološko preiskavo široke palete vzorcev. Specifičnost uvedenih metod je primerljiva s podatki v literaturi. Molekularnobiološke preiskave uporabljamo v diagnostiki limfomov od leta 1997.

Keywords

No keyword data available

Data

Language:	Slovenian
Year of publishing:	2008
Typology:	1.04 - Professional Article
Organization:	OI - Institute of Oncology
UDC:	616-006
COBISS:	25339865
ISSN:	1408-1741
Parent publication:	Onkologija
Views:	2274
Downloads:	538
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Molecular Diagnosis of Lymphomas
Secondary abstract:	Demonstration of clonality and detection of specific genetic abnormalities enable distinguishing between neoplastic lesions and reactive processes and, thus, have an important value in the diagnosis of lymphoid neoplasms. A clonal population of lymphoid cells can be detected by PCR amplification of rearranged genes encoding B- and T-cell receptors. Furthermore, chromosomal translocations specific for certain lymphomas can also be detected by PCR. We have introduced PCR-based assays for clonality analysis, and for the detection of t(11;14) and t(14;18) chromosome translocations in mantle-cell and follicular lymphoma, respectively. Assays are simple and fast and can be applied on small amounts of blood and tissue samples. Detection rates of our PCR assays are in agreement with reported data. We have been using molecular techniques as additional methods in the diagnosis of lymphoproliferative disorders since 1997.
URN:	URN:NBN:SI
Source comment:	BSDOCID143379;
Pages:	str. 119-121
Volume:	ǂLetn. ǂ12
Issue:	ǂšt. ǂ2
Chronology:	2008
ID:	10956166