diplomsko delo
Eva Miljevič (Author), Bojana Bogovič Matijašić (Reviewer), Sonja Smole Možina (Mentor)

Abstract

Zaradi neustrezne in nekritične uporabe protimikrobnih zdravil (PZ) pri živalih, ki vstopajo v prehransko verigo človeka, število odpornih bakterijskih sevov, osamljenih iz živali, kakor tudi živil, narašča. Kampilobakterioza, ki jo povzroča Campylobacter jejuni je ena izmed najpogosteje prijavljenih gastrointestinalnih bolezni pri ljudeh, večja prevalenca odpornih bakterijskih sevov pa povečuje tveganje za neučinkovito zdravljenje bolezni. Zato je pomembno na ta problem opozarjati vse deležnike živilske proizvodno-oskrbovalne verige in tudi zdravnike, da se redno izvajajo testi bakterijske odpornosti na najpomembnejša protimikrobna zdravila (PZ). V eksperimentalnem delu smo določali odpornost 8 sevov C. jejuni iz proizvodnega (klavniškega) in 8 sevov iz naravnega (površinskih voda) okolja proti šestim PZ (eritromicinu, ciprofloksacinu, tetraciklinu, gentamicinu, nalidiksni kislini in streptomicinu) na komercialno pripravljenih mikrotitrskih ploščicah SensititreTM EUCAMP2. Tako klavniški (KS) kot vodni (VS) sevi so pokazali odpornost proti istim vrstam PZ – ciprofloksacinu (CIP), tetraciklinu (TET) in nalidiksni kislini (NAL). Delež odpornih sevov proti posameznim PZ je bil višji pri klavniških (CIP 87,5 %, TET 50 %, NAL 50 %) kot vodnih (CIP 25 %, TET 12,5 %, NAL 25 %) sevih. Zasledili smo tudi večkratno odpornost, ki je bila pri klavniških sevih 87,5 % pri vodnih pa 25 %, in sicer med CIP in TET (KS: 37,5 %), ter CIP in NAL (KS: 50 %, VS: 25 %). Prišli smo do sklepa, da je vzrok večje odpornosti KS večja izpostavljenost PZ, vzrok odpornosti VS pa je verjetno prenos odpornih sevov iz klavnice v njeno okolico.

Keywords

Campylobacter jejuni;protimikrobna zdravila;odpornost proti antibiotikom;klavniški sevi;vodni sevi;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [E. Miljevič]
UDC: 579.2+579.6:615.33
COBISS: 4940408 Link will open in a new window
Views: 862
Downloads: 209
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Other data

Secondary language: English
Secondary title: Resistance of Campylobacter jejuni strains from the natural and production environment to antimicrobial drugs
Secondary abstract: Due to inadequate and uncritical use of antimicrobial drugs in animals, entering the human food chain, the number of resistant bacterial strains, isolated from humans as well as from the food, is increasing. Campylobacteriosis caused by Campylobacter jejuni is one of the most frequently reported gastrointestinal diseases in humans. The appearance of resistant bacterial strains increases the risk of ineffective treatment of the disease. Therefore, it is important to alert all stakeholders in the food production and supply chain, as well as doctors, to regularly carry out bacterial resistance tests on the most important antibiotics. In our experimental work, we tested the resistance of 8 strains of C. jejuni from the production (slaughterhouse) and 8 strains from the natural (surface water) environment against six antimicrobial drugs (erythromycin, ciprofloxacin, tetracycline, gentamicin, streptomycin nalidixic acids) on commercially prepared microtiter plates SensititreTM EUCAMP2. Both slaughtering (SS) and water (WS) strains have shown resistance to the same groups of antibiotics - ciprofloxacin (CIP), tetracycline (TET) and nalidixic acid (NAL). The proportion of resistant strains against individual AM was higher in slaughterhouse (CIP 87,5 %, TET 50%, NAL 50%) than water (CIP 25 %, TET 12,5 %, NAL 25 %) strains. Multidrug resistance was also present, which is in the case of slaughterhouse strains 87,5 % and 25 % in water strains, namely between CIP and TET (SS: 37,5 %) and CIP and NAL (SS: 50 %, WS: 25 %). We have come to the conclusion that the cause for the higher resistance levels of SS might be a higher exposure to antibiotics, and the cause of resistant WS is the transfer of resistant strains from the slaughterhouse to its surroundings.
Secondary keywords: Campylobacter jejuni;antimicrobial drugs;antimicrobial resistance;slaughter strains;water strains;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages: VIII, 27 f.
ID: 10958835