magistrsko delo
Katarina Šoln (Author), Matevž Likar (Reviewer), Jasna Dolenc Koce (Mentor), Jasna Dolenc Koce (Thesis defence commission member), Nada Žnidaršič (Thesis defence commission member), Matevž Likar (Thesis defence commission member), Simona Strgulc-Krajšek (Thesis defence commission member), Nada Žnidaršič (Co-mentor)

Abstract

Japonski in češki dresnik sta invazivni tujerodni rastlinski vrsti, ki s svojo hitro rastjo, vegetativnim razmnoževanjem in alelopatijo zavirata rast sosednjih rastlin. Namen naše raziskave je bil ugotoviti, kakšen je vpliv izvlečkov iz njunih korenik na strukturne in biokemijske značilnosti kalic vrtne redkvice. S tankoplastno kromatografijo smo ugotovili, da imata izvlečka obeh dresnikov podobno sestavo. Pri redkvici, ki smo jo izpostavili vodnim izvlečkom s koncentracijami od 0,5 do 10 %, se delež kaljivosti ni zmanjšal. Po 7 dneh kalitve so bile najbolj prizadete korenine, medtem ko poganjki niso bili prizadeti. Korenine so bile kratke in široke, na kar sta vplivali predvsem spremenjena oblika in velikost celic skorje. V tretiranih rastlinah je bila spremenjena tudi zgradba koreninske čepice in apikalnega meristema. Biokemijski pokazatelji oksidativnega stresa (encimski in neencimski antioksidanti, lipidna peroksidacija) so se spremenili večinoma neznačilno. Barvanje z diaminobenzidinom je pokazalo povečano prisotnost vodikovega peroksida v tretiranih kalicah. S presevnim elektronskim mikroskopom smo analizirali ultrastrukturo koreninskih vršičkov. Izvlečka dresnikov sta najbolj vplivala na celice koreninske čepice, v katerih smo opazili odstop celične membrane od celične stene, krpata jedra z zgoščenim heterokromatinom, obročaste mitohondrije in razširjene cisterne granularnega endoplazemskega retikuluma z gosto vsebino. Nekatere celice so že odmrle, pri drugih je struktura kazala na programirano celično smrt. Meristemske celice so bile manj prizadete kot koreninska čepica, prisotnih je bilo več vakuol.

Keywords

alelopatija;dresnik;izvleček;kalitev;mikroskopija;rast;struktura;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [K. Šoln]
UDC: 577.2(043.2)
COBISS: 4822351 Link will open in a new window
Views: 900
Downloads: 317
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Other data

Secondary language: English
Secondary title: Effect of japanese and bohemian knotweed rhizome extracts on germination, growth, morphological and biochemical characteristics of radish
Secondary abstract: Japanese and Bohemian knotweed are invasive alien plants that are known to inhibit the growth of their neighbors with quick growth, vegetative reproduction, and allelopathy. The aim of our study was to evaluate the effect of their rhizome extracts on morphological and biochemical characteristics of radish seedlings. Biochemical analysis of extracts with the thin layer chromatography revealed similar composition for both knotweeds. The germination rate of radish seeds did not decrease after the exposure to aqueous extracts with concentration from 0,5% to 10%. After 7 days of germination, radish roots were the most inhibited, whereas shoots were not affected. Roots were short and thick due to change of shape and size of cortex cells. In treated plants, the structure of the root cap and the apical meristem were changed. Biochemical characteristics of oxidative stress (enzymatic and non-enzymatic antioxidants, lipid peroxidation) were changed but mainly nonsignificantly. Staining with diaminobenzidine showed the increased synthesis of hydrogen peroxide in roots. Root tip ultrastructure was analyzed with transmission electron microscope. Knotweed extracts had a strong effect on root cap cells: cell membrane detached from the cell wall, irregularly shaped nuclei with condensed heterochromatin, ring-shaped mitochondria and round granular endoplasmic reticulum with dense contents were observed. Some cells were dead, in others signs of programmed cell death appeared. Meristem cells were less damaged than root cap, more vacuoles were presented.
Secondary keywords: allelopathy;knotweed;extract;germination;microscopy;growth;structure;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. Ljubljana, Biotehniška fak.
Pages: XI f., 93, [2] str.
ID: 10958845