diplomsko delo
Rebeka Strah (Author), Maruša Pompe Novak (Mentor), Maruša Pompe Novak (Thesis defence commission member), Mojca Narat (Thesis defence commission member), Polona Jamnik (Thesis defence commission member)

Abstract

Fitoplazme so rastlinski intracelularni paraziti, ki živijo v floemu in jih prenašajo žuželke s sesanjem okuženega floemskega soka. V Sloveniji fitoplazma 'Candidatus. Phytoplasma solani' povzroča na vinski trti bolezen počrnelosti lesa. Bolezenska znamenja se pojavijo po vsej rastlini zaradi fitoplazemskih efektorskih proteinov. Da bi bolje preučili naravo efektorskih proteinov 'Ca. P. solani', smo z agrotransformacijo rastlin Nicotiana benthamiana prehodno izrazili šest potencialnih efektorjev spojenih z rumenim fluorescenčnim proteinom in jih šestnajst ur opazovali pod konfokalnim makroskopom. Z ustreznim redčenjem bakterijskih kultur smo transformirali večino celic na listni površini in posameznih celic, v katerih razdelkih smo videli fluorescenco. Pričakovanega prehajanja proteinov med rastlinskimi celicami nismo opazili ne pri potencialnih efektorskih proteinih, ne pri kontrolnem zelenem fluorescentnem proteinu. Domnevamo, da je nezmožnost difuzije proteinov med celicami v našem sistemu posledica imunskega odziva rastline na okužbo z Aagrobacterium tumefaciens, potrebno za transformacijo. Poznavanje mehanizmov navadne trsne rumenice je bistveno za njeno preprečevanje in zatiranje, zato moramo poglobiti raziskave potencialnih efektorskih proteinov, ki jo povzročajo.

Keywords

fitoplazma;efektorski proteini;Candidatus Phytoplasma solani;Nicotiana benthamiana;prehodna transformacija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [R. Strah]
UDC: 602.6:582.930:632.3:577.21(043.2)
COBISS: 9054585 Link will open in a new window
Views: 1466
Downloads: 413
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Other data

Secondary language: English
Secondary title: Analysis of potential effectors of phytoplasma 'Candidatus Phytoplasma solani' in transformed Nicotiana benthamiana plants
Secondary abstract: The phytoplasma are plant intracellular parasites that live in the phloem and are transmitted by insects ingesting infected floem sap. In Slovenia the phytoplasma 'Candidatus Phytoplasma solani' causes Bois noir disease in grape vine. The symptoms appear across the whole plant due to phytoplasma effector proteins. To better understand the nature of effector proteins, we transiently expressed six potential effectors fused with yellow fluorescent protein in Nicotiana benthamiana plants with agrotransformation and observed them for sixteen hours with a confocal macroscope. With appropriate dilutions of the bacterial cultures we successfully transformed most cells and single cells on the leaf surface and observed fluorescence in their compartments. We did not observe the expected movement of proteins between the plant cells for the potential effector proteins and the control green fluorescent protein. We assume that the inability of protein diffusion between cells in our system is a consequence of an immune response of the plants to infection with Aagrobacterium tumefaciens, needed for the transformation. Understanding the mechanisms of Bois noir disease is crucial for its prevention and suppression; it is therefore necessary to increase the research of the potential effector proteins that cause it.
Secondary keywords: phytoplasma;effectro proteins;transient transformation;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 21 str., [7] str. pril.
ID: 10959156