magistrsko delo
Dina Ramić (Author), Barbara Kraigher (Reviewer), Sonja Smole Možina (Mentor)

Abstract

Medcelično signaliziranje uravnava različne lastnosti bakterij, med njimi tudi gibljivost in adhezijo. Obe lastnosti sta povezani s filmotvornostjo, ki je velik problem v živilsko-prehranski oskrbovalni verigi. Številni rastlinski pripravki delujejo protimikrobno in zavirajo tudi bakterijsko medcelično signaliziranje. Ker pa je učinek rastlinskih pripravkov na medcelično signaliziranje bakterij C. jejuni slabo raziskan, smo analizirali učinek 30 rastlinskih pripravkov na medcelično signaliziranje, gibljivost in adhezijo. Določili smo tudi korelacijo med spremembo medceličnega signaliziranja, gibljivosti in adhezije. Za ugotavljanje učinka rastlinskih pripravkov proti medceličnemu signaliziranju bakterij C. jejuni smo uporabili metodo merjenja bioluminiscence poročevalskega seva V. harveyi BB170 po dodatku izrabljenega gojišča C. jejuni, ki je bil gojen z ali brez dodatka rastlinskega pripravka v subinhibitorni koncentraciji (1/4 MIK). Večina rastlinskih pripravkov je zmanjšala medcelično signaliziranje C. jejuni, izmerjeno preko bioluminiscence, za več kot 80 %. Najboljši učinek je imel rastlinski metabolit tirosol (98 %). Testirali smo še učinek vseh pripravkov na gibljivost bakterij na poltrdnem gojišču. Pri 4 rastlinskih pripravkih (eterično olje navadnega brina (Juniperus communis), dobre misli (Origanum vulgare) in timijana (Thymus vulgaris), ter γ-terpinen) smo zaznali statistično značilen učinek na zmanjšanje gibljivosti. Potrdili smo statistično značilno korelacijo med spremembo medceličnega signaliziranja in gibljivosti. Ugotovili smo tudi, da 14 rastlinskih pripravkov statistično značilno zmanjšuje adhezijo bakterij C. jejuni na površino polistirenske mikrotitrske plošče, vendar statistično značilne korelacije med spremembo medceličnega signaliziranja in adhezije nismo potrdili.

Keywords

patogeni mikroorganizmi;Campylobacter jejuni;rastlinski pripravki;protimikrobne snovi;protibakterijska aktivnost;medcelično signaliziranje;gibljivost;adhezija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [D. Ramić]
UDC: 579.22/.26:547.9:615.33
COBISS: 4946552 Link will open in a new window
Views: 1320
Downloads: 566
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Other data

Secondary language: English
Secondary title: The effect of selected plant formulations on quorum sensing in Campylobacter jejuni
Secondary abstract: Quorum sensing (QS) regulates various types of bacterial behaviour, such as motility and adhesion to biotic and abiotic surfaces. Adhesion is the initial step in biofilm formation, which cause enormous problems in the food industry. Plant formulations have shown antibacterial activity and have also shown to be effective against QS. The effect of plant formulations on QS in C. jejuni has been poorly studied so far, so we tested the effect of 30 plant formulations on QS, motility and adhesion of these bacteria. We studied the correlation between the changes in QS, motility and adhesion. To determine the effect of plant formulations against QS in C. jejuni, we have used the bioluminiscence measurement of the reporter strain V. harveyi BB170, after addition of spent medium of C. jejuni, cultivated with or without addition of plant formulation in concentration of 1/4 MIC. Most of the plant formulations have reduced the AI-2 signal for more than 80 %, with the best effect of plant secondary metabolite tyrosol (98 %). Since plant formulations have shown excellent effect on QS, we tested their effect on bacterial motility on semisolid agar. 4 plant formulations reduced the bacterial motility with statistical significance (essential oils of juniperus (Juniperus communis), oregano (Origanum vulgare) and thyme (Thymus vulgaris), as well as γ-terpinene). We have shown statistically significant correlation between the changes in QS and motility. 14 plant formulations showed antiadhesion effects on C. jejuni on the surface of the polystyrene microtiter plate, but we have not confirmed statistically significant correlations between the changes in QS and adhesion.
Secondary keywords: pathogenic microorganisms;Campylobacter jejuni;plant formulations;antimicrobial compounds;antibacterial activity;quorum sensing;motility;adhesion;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: XII, 57 f., [3] f. pril.
ID: 10959417