diplomsko delo
Špela Kralj (Author), Hrvoje Petković (Mentor), Hrvoje Petković (Thesis defence commission member), Mojca Narat (Thesis defence commission member), Polona Jamnik (Thesis defence commission member)

Abstract

V pričujočem delu so predstavljeni osnovni principi afinitetne kromatografije, na podlagi katerih deluje ta ločevalna tehnika in možnosti njene uporabe. Ločevanje molekul je možno zaradi njihovih kemičnih in fizikalnih lastnosti, kar omogoča tvorbo specifičnih povezav z ligandi. To je osnova na podlagi katere so se razvili številni protokoli afinitetnega ločevanja. V tem diplomskem delu so predstavljeni različni nosilci in ligandi uporabljeni v ločevalnih kolonah. Opisanih je več tehnik afinitetne kromatografije – ločevanje proteinov na podlagi metilacijskih vzorcev pridobljenih v posttranslacijskih modifikacijah, ločevanje nekorirajoče RNA, ločevanje plazmidnih izoform in ločevanje na podlagi stereoizomerije. Številne možne kombinacije nosilcev, ligandov in pogojev kot so pH vrednost, ionska moč etc. uporabljenih med ločevanjem molekul omogočajo izredno aplikativnost uporabe afinitetne kromatografije za ločevanje strukturno najrazličnejših tarčnih molekul. Tehnika predstavlja zelo pomemben korak v čiščenju molekul tako v akademskem kot v industrijskem okolju.

Keywords

biotehnologija;afinitetna kromatografija;nosilci;ligandi;biotehnološke aplikacije;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Š. Kralj]
UDC: 602.1:543.544(043.2)
COBISS: 9065081 Link will open in a new window
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Downloads: 199
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Other data

Secondary language: English
Secondary title: Usage of affinity chromatography in biotechnology
Secondary abstract: Following work presents basic principles of affinity chromatography on which this separation technique is based and its application. This sepparation approach is based on physical and chemical properties of bioactive molecules, which specifically bind to target ligand. Based on this principle, a number of affinity separations procedures have been developed. Different separational matrices and ligands are reviewed in this seminar work.. Therefore, a number of affinity techniques are described such as protein separation approaches based on methylation post-translational modifications, separation of non-coding RNA, separation of plasmid isoforms and separation based on stereochemistry. Various combinations of matices, ligands and conditions like pH value, ionic strenght, etc. that can be used during separations of molecules are making this technique applicable to structurally diverse target molecules. Therefore, this technique presents very important step in purification at accademic as well as industrial environment.
Secondary keywords: biotechnology;affinity chromatography;matrixes;ligands;biotechnological applications;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 22 str.
ID: 10959918