magistrsko delo
Špela Gašperšič (Author), Rok Kostanjšek (Reviewer), Darja Keše (Mentor), Barbara Šoba (Co-mentor)

Abstract

Parazit Echinococcus granulosus sensu lato (s. l.) je trakulja, ki pri svojih vmesnih gostiteljih povzroča cistično ehinokokozo (CE). Končni gostitelj, običajno pes, v svojih iztrebkih izloča jajčeca trakulje, s katerimi se okužijo njeni vmesni gostitelji, govedo, ovce, koze, prašiči, konji, lahko tudi ljudje. Ob okužbi z jajčeci se v vmesnih gostiteljih, najpogosteje v njihovih jetrih, tvorijo hidatidne ciste. Za razumevanje epidemioloških značilnosti in prenosa trakulje se poslužujemo genotipizacijskih metod, s katerimi poskušamo ugotoviti genotip trakulje. V našo raziskavo smo vključili 15 punktatov ehinokoknih cist bolnikov in 5 ehinokoknih cist prašičev, pri katerih je bila ugotovljena CE. Echinococcus granulosus s. l. smo genotipizirali na podlagi pomnoževanja in sekvenčne analize delov genov cox1, nad1, nad5, rrnS in rrnL mitohondrijskega genoma trakulje in dveh delov jedrnega gena za 18S rRNA. Trakuljo smo uspešno genotipizirali pri 10/15 (66,7 %) bolnikih in pri 5/5 (100 %) prašičih. Med uspešno genotipiziranimi vzorci ehinokoknih cist bolnikov s CE smo pri 5/10 (50 %) ugotovili E. granulosus genotip G1, pri 1/10 (10 %) E. granulosus genotip G3 in pri 4/10 (40 %) E. canadensis genotip G7. Pri vzorcih ehinokoknih cist prašičev smo ugotovili E. canadensis genotip G7, ki je značilen za tega vmesnega gostitelja trakulje.

Keywords

trakulja;Echinococcus granulosus sensu lato;cistična ehinokokoza;genotipizacijske metode;verižna reakcija s polimerazo;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL MF - Faculty of Medicine
Publisher: [Š. Gašperšič]
UDC: 616.995.121:616-036.22:575.22
COBISS: 5040504 Link will open in a new window
Views: 1388
Downloads: 333
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Other data

Secondary language: English
Secondary title: Genotyping of Echinococcus granulosus
Secondary abstract: Echinococcus granulosus sensu lato (s.l.) is a tapeworm, which causes cystic echinococcosis (CE). It dwells in small intestines of definitive hosts, where it produces eggs, which are excreted into the environment. Intermediate hosts, such as sheep, cattle, pigs, goats, horses and humans become infected with ingestion of food, contaminated with eggs. After the infection, the larval stage results in the formation of echinococcal cysts, which are located mainly in liver. For better understanding of tapeworm transmission and epidemiological features, genotype of tapeworm is determined with genotyping methods. In this study we used 15 samples of echinococcal cysts obtained from patients with CE, and 5 samples obtained from pigs with CE. Genotyping was performed with amplifying and sequencing of mitochondiral (cox1, nad1, nad5, rrnS and rrnL) and nuclear genes (18S rRNA). Genotype was successfully determined in 10/15 of samples, obtained from patients (66,7 %) and 5/5 samples, obtained from pigs. From successfully genotyped samples from patients we determined E. granulosus genotype G1 in 5/10 (50 %) samples, E. granulosus genotype G3 in 1/10 (10 %) samples and E. canadensis genotype G7 in 4/10 (40 %) samples. In all echinococcal cysts samples, obtained from pigs, we determined E. canadensis genotype G7, which is distinctive causative agent for CE in pigs.
Secondary keywords: tapeworm;Echinococcus granulosus sensu lato;cystic echincoccosis;genotyping methods;polymerase chain reaction;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: XI, 67 f.
ID: 11080202