magistrsko delo
Tilen Notersberg (Author), Damjana Drobne (Reviewer), Romana Marinšek-Logar (Mentor), Tina Eleršek (Co-mentor)

Abstract

Namen te magistrske naloge je bil ugotoviti vpliv bisfenola A in F na stopnjo rasti evkariontske mikroalge Pseudokirchneriella subcapitata in prokariontske cianobakterije Synechococcus leopoliensis. Mikroalge in cianobakterije smo za tri dni izpostavili različnim koncentracijam bisfenola A in F. Vsak dan smo izmerili gostoto celic s pretočnim citometrom, na koncu poskusa pa smo še s plinsko kromatografijo sklopljeno z masno spektrometričnim detektorjem preverili, če se je tekom poskusa v gojišču spremenila koncentracija testiranih bisfenolov. Ugotovili smo, da je zaviranje rasti odvisno od koncentracije bisfenolov. Za algo P. subcapitata smo izmerili naslednje ekotoksikološke podatke: EC50 = 6,8 mg/l, NOEC = 4,9 mg/l, LOEC = 15,6 mg/l za BPA in EC50 = 9,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l za BPF. Za cianobakterijo S. leopoliensis pa smo izmerili EC50 = 4,8 mg/l, NOEC = 0,5 mg/l, LOEC = 1,5 mg/l za BPA in EC50 = 5,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l za BPF. Rezultati kažejo, da ima BPF podobne učinke na alge in cianobakterije kot BPA in zato z okoljskega stališča ni primerna zamenjava za BPA.

Keywords

alge;mikroalge;cianobakterije;Pseudokirchneriella subcapitata;Synechococcus leopoliensis;bisfenol A;bisfenol F;rast;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [T. Notersberg]
UDC: 502/504:582.26:579.24
COBISS: 5059448 Link will open in a new window
Views: 655
Downloads: 221
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Other data

Secondary language: English
Secondary title: ǂThe ǂinfluence of bisphenol A in F on algal and cyanobacterial growth
Secondary abstract: The purpose of this masters thesis was to find out the effect of bisphenol A and F on the growth rate of eukaryotic microalgae Pseudokirchneriella subcapitata and prokaryotic cyanobacteria Synechococcus leopoliensis. Microalgae and cyanobacteria were exposed to different concentrations of bisphenol A and F for three days. During incubation, every day cell density was measured with a flow cytometer and, at the end of the experiment, bisphenol concentration was determined by gas chromatography coupled with a mass spectrometric detector to determine if their concentration changed during the experiment. We found that growth inhibition is concentration dependent. We obtained the following ecotoxicological values for P. subcapitata EC50 = 6,8 mg/l, NOEC = 4,9 mg/l, LOEC = 15,6 mg/l for BPA and EC50 = 9,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l for BPF. For S. lepopoliensis the values were EC50 = 4,8 mg/l, NOEC = 0,5 mg/l, LOEC = 1,5 mg/l for BPA and EC50 = 5,2 mg/l, NOEC = 2,9 mg/l, LOEC = 9,4 mg/l for BPF. Our result show that BPF has similar effects on algae and cyanobacteria as BPA and is therefore not a suitable replacement for BPA from an environmental point of view.
Secondary keywords: algae;microalgae;cyanobacteria;Pseudokirchneriella subcapitata;Synechococcus leopoliensis;bisphenol A;bisphenol F;growth;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: VIII, 47 f.
ID: 11155048