magistrsko delo
Jure Mravlje (Author), Alenka Gaberščik (Reviewer), Marjana Regvar (Mentor), Marjana Regvar (Thesis defence commission member), Matevž Likar (Thesis defence commission member), Alenka Gaberščik (Thesis defence commission member), Katarina Vogel-Mikuš (Thesis defence commission member), Matevž Likar (Co-mentor)

Abstract

Območje Žerjava je prekomerno onesnaženo s kovinami v tleh (svinec, kadmij, cink) kot posledica dolgoletnega obratovanja talilnice svinca, ki je močno vplivala tudi na okoliško vegetacijo. Znano je, da asociacije rastlin z glivnimi endofiti ublažijo stres in s tem izboljšajo preživetje rastlin v ekstremnih okoljih. Nabrali smo vzorce korenin treh drevesnih vrst (vrbe ive, navadne breze in rdečega bora) ter pripadajoče vzorce rizosfernih tal na dveh različno onesnaženih vzorčnih ploskvah (P2 in P3). Vzorcem tal smo določili vsebnost organske snovi, rastlinam dostopnega fosforja, celokupne koncentracije kovin in pH vrednost. Na koreninskih vzorcih drevesnih vrst smo ocenili kolonizacijo z ektomikoriznimi (EM) in arbuskularno mikoriznimi (AM) glivami ter temnimi septiranimi endofiti (DSE). Pri vseh drevesnih vrstah na obeh vzorčnih ploskvah je prevladovala kolonizacija z EM glivami. Potrdili smo pozitivno korelacijo med kolonizacijo z DSE in koncentracijami kovin v tleh. Izolirali smo DNA iz posameznih EM morfotipov in s pomočjo molekularnih metod (PCR, sekvenciranje) identificirali glivne simbionte pri posameznih drevesnih vrstah. Pri vrbi so prevladovale glive iz družine Sordariaceae, pri brezi glive iz družine Thelephoraceae, pri boru pa glive iz družine Pyronemataceae. Ugotovili smo, da so razlike v mikorizni kolonizaciji in strukturi glivnih simbiontov bolj pogojene z drevesno vrsto kot z vzorčno ploskvijo (stopnjo onesnaženja).

Keywords

ektomikoriza;arbuskularna mikoriza;temni septirani endofiti;onesnaženo območje;prekomerne koncentracije kovin;PCR;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [J. Mravlje]
UDC: 577(043.2)
COBISS: 5106511 Link will open in a new window
Views: 1484
Downloads: 415
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Other data

Secondary language: English
Secondary title: Root colonisation and characterisation of fungal endophytes of selected tree species growing at metal enriched site in Žerjav
Secondary abstract: Long-term lead mining and smelting activities in Žerjav area has resulted in global metal pollution and degradation of environment. Associations of plants with fungal endophytes may alleviate stress and improve survival of plants in extreme environments. The root samples of three different tree species (goat willow, silver birch and scots pine) and their rhizosphere soils were collected at two different sampling sites (P2 and P3). The total organic content, plant accesible phosphorus, the total metal concentration and pH were measured on soil samples. Colonisation with ectomycorrhizal (EM), arbuscular mycorrhizal (AM) and dark septate endophytes (DSE) was evaluated. EM colonisation dominated in all tree species at both sampling sites. Positive correlation between the level of DSE colonisation and increased soil metal concentration was confirmed. DNA was isolated from different EM morphotypes. Fungal symbionts were identified with molecular methods (PCR, sequencing). Fungi from family Sordariaceae were the most abudant in goat willow, Thelephoraceae dominated in silver birch and Pyronemataceae were the most common in scots pine. The results show that tree species is more important factor determining mycorrhizal colonisation and fungal community structure than the sampling site (degree of pollution).
Secondary keywords: ectomycorrhiza;arbuscular mycorrhiza;dark septate endophytes;polluted area;metal enriched area;PCR;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. Ljubljana, Biotehniška fak.
Pages: XII, 78, [8] f.
ID: 11159367