Adhezija bakterij na izbrane lesne premaze

diplomsko delo

David Kekec (Author), Miha Humar (Reviewer), Marko Petrič (Mentor), Klemen Bohinc (Co-mentor)

Abstract

Bakterijska adhezija na površinah in pozneje nastanek biofilma predstavljata glavni vzrok kroničnih okužb z negativnim vplivom na zdravje ljudi. S tem problemom se srečujemo tudi pri izbiri in izdelavi pohištva, kjer stremimo k razvoju izdelkov s takšnimi površinami, na katere naj se bakterije ali drugi mikroorganizmi praviloma ne bi oprijemali. Predmet naše raziskave je bil testirati izbrana premazna sredstva za les (laneno olje, vodna lazura, nitrocelulozni lak, nanopremaz) na adhezijo bakterij vrste Pseudomonas aeruginosa, kjer smo kot testno površino uporabili vezano bukovo ploščo. Topografijo testne površine in število oprijetih bakterij P. aeruginosa smo opazovali s SEM. Na podlagi kvantitativne analize SEM slik smo določili število oprijetih bakterij z obkroževanjem le-teh. Preliminarne rezultate smo predstavili v obliki posnetkov SEM. Rezultati nakazujejo, da se je največje število bakterij oprijelo na površini, premazani z lanenim oljem. Ugotovili smo, da lahko s SEM mikroskopom ob ustrezni metodologiji dela opazujemo adhezijo bakterij na lesenih površinah. Za potrditev in pridobitev relevantnih rezultatov bodo v prihodnosti potrebne obširnejše in bolj poglobljene študije.

Keywords

vezana bukova plošča;adhezija bakterij;Pseudomonas aeruginosa;lesni premazi;

Data

Language:	Slovenian
Year of publishing:	2019
Typology:	2.11 - Undergraduate Thesis
Organization:	UL BF - Biotechnical Faculty
Publisher:	[D. Kekec]
UDC:	630*829:832.282
COBISS:	3084425
Views:	890
Downloads:	195
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Bacterial adhesion to sellected wood finishes
Secondary abstract:	Bacterial adhesion to surfaces and subsequent biofilm formation are the main causes of chronic infections with a negative impact to the human health, and this is a problem in the process of manufacturing furniture. We this aimed to develop antibacterial surfaces to which the microorganisms do not adhere. The focus of our research was to test selected wood coatings (linseed oil, water-borne stain, nitrocellulose varnish, nano-coating) with regard to the adhesion of Pseudomonas aeruginosa, where we used a beech plywood as the test surface. The topography of the test surface and number of adhering bacteria were observed on SEM micrographs. The number of adhering bacteria was determined using quantitative analysis of the SEM images. Preliminary results were presented in the form of SEM recordings. The results indicate that the maximum number of bacteria adhered to the surface coated with linseed oil. We found that by using an SEM microscope, and the appropriate methodology of work, we can observe the adhesion of bacteria on wooden surfaces. In the future, extensive and in-depth studies will be needed to confirm and obtain the relevant results.
Secondary keywords:	plywood;bacterial adhesion;Pseudomonas aeruginosa;wood finishes;
Type (COBISS):	Bachelor thesis/paper
Study programme:	0
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Biotehniška fak., Oddelek za lesarstvo
Pages:	IX, 41 f.
ID:	11192187