Ekstrakcija artemizinina iz sladkega pelina Artemisia annua L.

diplomsko delo univerzitetnega študijskega programa I. stopnje

Anja Hribernik (Author), Maša Knez Hrnčič (Mentor), Petra Kotnik (Co-mentor), Darija Cör (Co-mentor)

Abstract

Sladki pelin je trenutno ena izmed najbolj iskanih zdravilnih rastlin. Najpomembnejša zdravilna učinkovina, ki jo vsebuje, je artemizinin; antimalarik. Skupaj z železom tvori kisikove radikale, ki učinkovito zatirajo parazite. Vsebuje tudi antioksidante. Cilj diplomskega dela je bil ugotoviti vpliv ekstrakcijskega postopka ter vpliv različne letine materiala na vsebnost artemizinina v ekstraktih. Spremljali smo tudi kinetiko ekstrakcije. Določiti smo želeli optimalne obratovalne pogoje za pridobitev ekstraktov s čim višjo vsebnostjo artemizinina. Kot ekstrakcijski medij smo poleg konvencionalnih topil uporabili tudi superkritične fluide. Primerjali smo sladki pelin dveh različnih letin ter raziskali, kako čas žetve vpliva na vsebnost artemizinina. Izvajali smo ekstrakcijo s hladnim topilom (maceracija) in ultrazvočno ekstrakcijo z etanolom ter mešanico topil heksan - etil acetat in ekstrakcijo s superkritičnimi fluidi; kot topilo smo uporabili superkritični CO2. Sledila je karakterizacija ekstraktov s tekočinsko kromatografijo z masno spektrometrijo (LC-MS/MS). Ugotovili smo, da je učinkovitost ekstrakcije odvisna od vrste materiala, ekstrakcijskega postopka in izbire topila. Vsebnost artemizinina v ekstraktih sladkega pelina pobranega v različnih časovnih obdobjih je bila različna, kar je posledica razpadanja sekundarnih metabolitov s časom. Izkoristki in vsebnost artemizinina v ekstraktih pridobljenih v vseh topilih (etanol, mešanica topil: 95 % n-heksan, 5 % etil acetat in ogljikov dioksid) so bili primerljivi, zato lahko sklepamo, da so vsa topila primerna za izolacijo artemizinina iz sladkega pelina. Vendar pri superkritični ekstrakciji ni potrebno nakdnadno čiščenje ekstraktov, zato je možnost, da bi artemizinin pri odstranjevanju topila razpadel, manjša.

Keywords

Artemisia annua L.;artemizinin;antimalarik;izolacija;kromatografija;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2019
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[A. Hribernik]
UDC:	543.544.19:582.991.15(043.2)
COBISS:	22613014
Views:	1335
Downloads:	261
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Extraction of artemisinin from Artemisia annua L.
Secondary abstract:	Artemisia annua L. is currently one of the most sought after medicinal plants. The most important active substance it contains is artemisinin, an antimalaric. Together with iron, it forms oxygen radicals that effectively suppress parasites. It also contains antioxidants. The objective of the thesis was to determine the effects of the extraction process and the effects of different harvest material on the content of artemisinin in the extracts. We also monitored the extraction kinetics. We wanted to determine the optimum operating conditions for obtaining extracts with the highest artemisinin content. In addition to conventional solvents, supercritical fluids were also used as the extraction medium. We compared the wormwood from two different years of harvests and investigated how harvest time affects artemisinin content. Cold solvent extraction (maceration) and ultrasonic extraction with ethanol and solvent mixture of hexane-ethyl acetate and extraction with supercritical fluids were performed; supercritical CO2 was used as the solvent. Characterization of the extracts by liquid chromatography by mass spectrometry (LC-MS/MS) followed. We have found that extraction efficiency depends on the type of material, the extraction process and the choice of solvent. The content of artemisinin in the extracts of Artemisia annua L. picked at different time periods varied as a result of the decay of secondary metabolites over time. The yields and the content of artemisinin in the extracts obtained in all solvents (ethanol, solvent mixture: 95% n-hexane, 5% ethyl acetate and carbon dioxide) were comparable, so it can be concluded that all solvents are suitable for the isolation of artemisinin from Artemisia annua L. However, supercritical extraction does not require the subsequent purification of the extracts, so the possibility of artemisinin decaying when the solvent is removed is less.
Secondary keywords:	Artemisia annua L.;artemisinin;antimalaric;isolation;chromatography;
Type (COBISS):	Bachelor thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	VIII, 36 str.
ID:	11207839