Antioksidativni potencial konopljinih semen

diplomsko delo

Urška Šetina (Author), Tomaž Požrl (Reviewer), Helena Abramovič (Mentor)

Abstract

Konopljina semena, katerih priljubljenost vse bolj narašča, so izvrsten vir hranilnih snovi, esencialnih aminokislin in maščobnih kislin. Vsebujejo tudi nekatere bioaktivne molekule, ki delujejo kot antioksidanti in imajo pozitiven vpliv na zdravje človeka. Cilj diplomske naloge je bil določiti vsebnost fenolnih spojin v različnih sortah konopljinih semen. Prav tako smo določali antioksidativni potencial (AOP) konopljinih semen tako v homogenem (etanol), kot tudi heterogenem (emulzija linolne kisline v vodi) okolju. Semena smo zmleli in razmastili ter ekstrahirali fenolne spojine s 70 % etanolom. Določali smo skupne fenolne spojine s Folin Ciocalteujevo metodo, sposobnost lovljenja prostih radikalov DPPH• in izvedli metodo beljenja ?-karotena v emulziji. Ugotovili smo, da se učinkovitost posameznih antioksidantov razlikuje v različnih sistemih. Prav tako smo prišli do zaključka, da sorte z največ fenolnih spojin nimajo največjega AOP.

Keywords

industrijska konoplja;Cannabis sativa L.;konopljina semena;antioksidanti;fenolne spojine;antioksidativni potencial;

Data

Language:	Slovenian
Year of publishing:	2019
Typology:	2.11 - Undergraduate Thesis
Organization:	UL BF - Biotechnical Faculty
Publisher:	[U. Šetina]
UDC:	633.522:66.094.3.097.8:547.56
COBISS:	5087352
Views:	817
Downloads:	230
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Antioxidant potential of hemp seeds
Secondary abstract:	Hemp seeds are an excellent source of nutrients, such as essential amino acids and fatty acids, and their popularity is growing every day. They also contain bioactive molecules that function as antioxidants and therefore have a positive impact on human health. The aim of graduation thesis was to determine content of total phenolic compounds in different sorts of hemp seeds. We have also examined antioxidant potential (AOP) in different systems: homogeneous (ethanol) and heterogeneous (emulsion of linoleic acid in water) system. Before the extraction of phenolic compounds in 70 % ethanol, hemp seeds were grinded and defatted. We determined total phenolic content with Folin Ciocalteu method, analysed the scavenging activity of free DPPH• radicals and carried out ?-carotene bleaching assay in emulsion. We came to the conclusion that activity of particular antioxidant in homogeneous and heterogeneous system differentiates. Furthermore, hemp seeds with the highest content of phenolic compounds do not have the highest AOP.
Secondary keywords:	industrial hemp;Cannabis sativa L.;hempseed;antioxidants;phenolic compounds;antioxidant potential;
Type (COBISS):	Bachelor thesis/paper
Study programme:	0
Thesis comment:	Univ. v Ljubljani, Biotehniška fak., Oddelek za živilstvo
Pages:	VIII, 22 f.
ID:	11212344