Protibakterijska terapija z bakteriofagi

diplomsko delo

Tena Janežič (Author), Matej Butala (Mentor), Matej Butala (Thesis defence commission member), Polona Jamnik (Thesis defence commission member), Mojca Narat (Thesis defence commission member)

Abstract

Bakteriofagi so virusi, katerih gostitelji so bakterije. Porast na antibiotike odpornih bakterij ki povzročajo okužbe je pripomogel k raziskovanju alternativnih oblik zdravljenja okužb. Terapija z uporabo bakteriofagov se je v dosedanjih raziskavah pokazala za uspešno. V naravi predvidoma lahko najdemo bakteriofage za vsako vrsto bakterij. Zaradi svoje specifičnosti do gostitelja lahko bakteriofagi ciljano uničijo tudi le določen sev bakterij. Da bi izboljšali učinkovitost zdravljenja z bakteriofagi, za namen terapije pripravimo bakteriofagni koktejl, ki vsebuje več različnih virusov, lahko pa določene bakteriofage tudi modificiramo in s tem spremenimo spekter gostiteljev. To lahko dosežemo tako, da v genom bakteriofaga vnesemo določene gene z zapisom za proteine s katerimi bakteriofag lažje lizira celico ali pa poruši biofilm bakterij in tako v kombinaciji z antibiotiki učinkoviteje odstranimo patogena. Tretma kombinacije bakteriofagov in antibiotikov se je izkazal za učinkovitejšo kot samostojna terapija z omenjenima sredstvima. Na bakteriofag konjugirani antibiotiki so se izkazali za zelo uspešno obliko zdravljenja okužb, saj se tako na mestu okužbe zaradi specifičnosti bakteriofagov do gostitelja koncentracija antibiotikov poviša. Za uveljavitev terapije z bakteriofagi v medicinski stroki, je potrebno opraviti raziskave o varnosti in zgradbi bakteriofagnih koktejlov, prav tako pa je potrebno tudi urediti regulacijske zahteve na tem področju.

Keywords

bakteriofag;fag;bakterije;protibakterijska terapija;

Data

Language:	Slovenian
Year of publishing:	2019
Typology:	2.11 - Undergraduate Thesis
Organization:	UL BF - Biotechnical Faculty
Publisher:	[T. Janežič]
UDC:	602.3:578.347:606:616-022(043.2)
COBISS:	9365625
Views:	1029
Downloads:	253
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Antibacterial bacteriophage therapy
Secondary abstract:	Bacteriophages are viruses which infect bacteria. Rise of antibiotic-resistant bacteria has caused an increase in research in the field of alternative therapies against pathogenic bacteria. Bacteriophage therapy has shown a great potential in treating infections. In the nature bacteriophage exist that can virtually infect every known bacterial species. Because of their specificity for a certain host, bacteriophages can infect and kill even only one strain of a bacterium. In order to improve efficacy of phage therapy, diverse phages can be mixed into cocktails containing three or more different phages. This prevents, that resistance development against one phage would decrease the efficiency of the phage therapy. Phages can also be modified, thus we can insert specific genes into their genomes, thus engineered phages can target selected bacterial strain or easier lyse bacterial cell or disperse biofilms. Combining phage therapy and antibiotic therapy showed big improvements in the treatment of infections, exhibiting greater efficacy of the therapy than with bacteriophage or antibiotics alone. In addition, antibiotics that are conjugated to phages are succesfully dellivered to the site of infection because of bacteriophage specificity, providing higher local concentration of antibiotics at the site of infection. For the use of phage therapy in every day clinical practice safety, efficiacy and composition of phage cocktails must be precisely investigated, also regulations in the field of using bacteriophages as medicinal agents must be carefully examined and adjusted to enable the prudent use of such therapy in clinics.
Secondary keywords:	bacteriophage;phage;bacteria;antibacterial therapy;
Type (COBISS):	Bachelor thesis/paper
Study programme:	0
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages:	VI, 20 str.
ID:	11217167