Ionska kromatografija za določevanje anionov

diplomsko delo

Rok Tomšič (Author), Mitja Kolar (Mentor)

Abstract

V okviru diplomskega dela na Fakulteti za kemijo in kemijsko tehnologijo, Univerze v Ljubljani, je bil vpeljan sistem za anionsko izmenjevalno kromatografijo s konduktometrično detekcijo. S sistemom so bile izvedene analize realnih vzorcev v katerih smo določali: fluoridne, kloridne, nitritne, bromidne, nitratne, fosfatne in sulfatne ione. Metoda ionske kromatografije je bila tudi delno validirana, saj smo študirali linearnost ter določili LOD in LOQ. Določali smo primernost oz. vpliv kromatografskih pogojev kot so: pretok in sestava, pH ter starost eluenta. Eksperimentalno delo je vključevalo tudi regeneracijo kolone, s katero smo želeli doseči manjšo asimetričnost vrhov, ustrezno ločitev vrhov, ki pripadata fosfatnim in sulfatnim ionom ter odpraviti zamike retencijskih časov.

Keywords

analizna kemija;analizne metode;kromatografske metode;ionska kromatografija;anioni;validacija;regeneracija kolone;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2019
Typology:	2.11 - Undergraduate Thesis
Organization:	UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher:	[R. Tomšič]
UDC:	543.544.14(043.2)
COBISS:	1538327747
Views:	1357
Downloads:	470
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary abstract:	As a part of the diploma thesis at the Faculty of Chemistry and Chemical Technology of the University of Ljubljana, an anion exchange chromatography system with conductometric detection was introduced. The system performed analyses of real samples in which we determined: fluoride, chloride, nitrite, bromide, nitrate, phosphate and sulphate ions. The ion chromatography method was also partially validated, as we studied linearity and determined LOD and LOQ. We determined the suitability or the influence of the chromatographic conditions such as: flow and composition, pH and age of the eluent. The experimental work also included the regeneration of the column, which was aimed at reducing the peak asymmetry of the peaks, the proper separation of the peaks belonging to the phosphate and sulphate ions, and to eliminate the retention time delays.
Secondary keywords:	ion chromatography;anions;validation;column regeneration;
Type (COBISS):	Bachelor thesis/paper
Study programme:	1000374
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, VSŠ Kemijska tehnologija
Pages:	XIV, 38 str.
ID:	11217768