magistrsko delo
Amadeja Lapornik (Author), Tatjana Avšič-Županc (Mentor), Vera Župunski (Thesis defence commission member), Marko Dolinar (Thesis defence commission member)

Abstract

Peščene muhe so pomembni prenašalci bakterij, virusov in parazitov v Sredozemlju, Afriki, na Bližnjem vzhodu ter v Aziji. V Evropi je več vrst peščenih muh. Aktivnost muh je večja v toplejših mesecih, zato se zaradi podnebnih sprememb in segrevanja ozračja njihov življenjski prostor širi. Vsaj devet vrst peščenih muh prenaša fleboviruse, ki spadajo v družino Phenui viridae. Okužbe s flebovirusi so nevarne za človeka in živali. Največkrat potekajo brez hujših zapletov, z osnovnimi znaki okužb kot so bruhanje, glavobol in povišana telesna temperatura. Virus Toscana lahko pri človeku povzroči tudi meningitis in nevrološke zaplete. Fleboviruse so že dokazali na območjih s podobnim podnebjem kot je v Sloveniji. Tudi pri nas beležimo naraščajoče število okužb, kljub temu je prevalenca flebovirusov v prenašalcih še neraziskana. Z magistrsko nalogo smo želeli vpeljati molekularne postopke za odkrivanje flebovirusov v peščenih muhah, ki smo jih vzorčili na številnih lokacijah po Sloveniji. Kljub temu, da smo z vpeljanimi molekularnimi metodami uspeli specifično pomnoževati tarčne odseke različnih flebovirusov, ki so jih že opisali v sosednjih državah, v vzorcih peščenih muh, ki smo jih ulovili v sezoni 2017 in 2018 v Sloveniji, nismo odkrili flebovirusov.

Keywords

virologija;flebovirusi;okužbe;prenašalci flebovirusov;peščene muhe;Slovenija;verižna reakcija s polimerazo;PCR;optimizacija;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [A. Lapornik]
UDC: 578(043.2)
COBISS: 1538445763 Link will open in a new window
Views: 581
Downloads: 123
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Other data

Secondary language: English
Secondary title: Probing Phleboviruses in sandfly captured in selected locations in Slovenia
Secondary abstract: Sandflies (Phlebotomus spp.) are the most important carriers of viruses and parasites in the Mediterranean, Africa, Middle East and Asia. In Europe, there are several sandfly species and each has a different competence for transmitting vector-borne diseases. Fly activity is higher in warmer months and because of climate changes their habitat is expanding. Sandflies are important vectors of phleboviruses belonging to the Phenuviridae family, order Bunyavirales. In humans and animals, infections with phleboviruses are most often associated with a symptomatic disease similar to influenza. Infection with a Toscana virus can also result in meningitis or severe neurological complications in humans. Phleboviruses have already been described in neighbouring countries with a similar climate. There is also an increasing number of phlebovine infections in Slovenia, but the frequency of infections of the vectors is still unknown. The aim of our study was to introduce and to optimize molecular protocols for specific detection of phleboviruses in different locations in Slovenia. Although we successfully introduced and optimized specific molecular diagnostic protocols for the detection of phleboviruses in sandfly samples, all tested samples, collected in Slovenia in 2017 and 2018 were found negative.
Secondary keywords: phleboviruses;Phlebotomus;Slovenia;PCR;optimisation;
Type (COBISS): Master's thesis/paper
Study programme: 1000377
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, smer Biokemija
Pages: 66 str.
ID: 11231238