magistrsko delo
Sandra Štruc (Author), Tadeja Matos (Reviewer), Eva Ružić-Sabljić (Mentor), Tjaša Cerar (Co-mentor)

Abstract

Lymska borelioza (LB) je zoonoza, ki jo povzroča spiroheta Borrelia burgdorferi sensu lato (Bb-sl). Borelije se prenašajo s pikom ščitastega klopa (Ixodes ricinus), ki se okuži pri sesanju krvi prosto živečih in domačih živali. V Sloveniji je LB endemična. LB pri psih se klinično težko opredeli, saj se kaže neznačilno, npr. kot poslabšanje splošnega stanja z anoreksijo in zvišano telesno temperaturo. Okužba pri psih se najpogosteje opredeli šele z dokazom specifičnih protiteles. V nalogi smo primerjali dve metodi, in sicer imunofluorescenčni test (IFT) in imunoblot (IB) test, s katerima smo dokazovali za borelije specifična protitelesa IgG. V raziskavo je bilo vključenih 100 vzorcev serumov oz. plazme psov s sumom na LB. Namen je bil dokazati prisotnost protiteles proti LB z obema metodama, primerjati rezultate, ter ugotoviti na katere borelijske antigene se pes odzove. Z IB je bilo pozitivnih 22 % serumov in 1 % serumov je imel mejno vrednost, medtem ko je bilo pri IFT pozitivnih 36 % serumov. Največja statistično značilna razlika v odstopnju obeh testov se je pokazala pri IFT titru 64, kjer je bilo z IFT pozitivnih 12 vzorcev in z IB negativnih 11 vzorcev ter eden pozitiven. Manjša statistično značilna razlika se je pojavila pri titru 128, kjer je bilo z IFT devet vzorcev pozitivnih in z IB sedem vzorcev negativnih ter dva pozitivna. Pri najvišjih titrih (256 in 512) so bili rezultati obeh testov enotni, pri titrih, nižjih od 64 pa razlike med testoma niso bilo statistično značilne. Pri IB smo najpogosteje ugotovili reakcijo na VlsE antigen.

Keywords

Borrelia burgdorferi sensu lato;lymska borelioza;serološke metode;indirektni imunofluorescenčni test;imunoblot;specifična protitelesa IgG;psi;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL MF - Faculty of Medicine
Publisher: [S. Štruc]
UDC: 579.62:616.993-078
COBISS: 5105272 Link will open in a new window
Views: 1084
Downloads: 233
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Other data

Secondary language: English
Secondary title: Comparison of two serological tests for confirmation Lyme borreliosis in dogs
Secondary abstract: Lyme borreliosis (LB) is a zoonotic disease, caused by spirohete Borrelia burgdorferi sensu lato (Bb-sl). It is transmitted through the bite of infected tick (Ixodes ricinus), which feeds on animals. In Slovenia, LB is endemic. LB in dogs is clinically difficult to identify, as it is not typical, and may appear as anorexia and fever. The infection in dogs is most often determined by the evidence of specific antibodies. In the master's thesis, two methods were compared: the immunofluorescence test (IFT) and the immunoblot (IB) with which we proved specific IgG antibodies. In the study we included 100 dog blood samples suspected of LB. The purpose was to demonstrate the presence of anti-Lyme borreliosis antibodies using both methods, compare results, and determine the response of the borrelia antigens in dogs. We have found, that with IB was 22 % of samples positive and 1 % borderline, in the meantime in the IFT 36 % was sera negative. Statistically significant difference between both tests were shown at IFT titer 64, where with IFT 12 samples were positive, while with IB 11 samples were negative and one positive. A smaller significant difference among results appeared at titer 128, where with IFT nine samples were positive, while with IB seven samples were negative and two positive. At the highest titres (256 in 512), the results were evaluated equally, with titers lower than 64 differences between tests were not statistically significant. At IB, the most commonly found was the reaction to antigen VlsE.
Secondary keywords: Borrelia burgdorferi sensu lato;Lyme borreliosis;serological methods;indirect immunofluorescence test;immunoblot assay;specific IgG antibodies;dogs;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Pages: IX, 42 f.
ID: 11238025