doktorska disertacija
Manca Pajnič (Author), Veronika Kralj-Iglič (Mentor), Andrej Starc (Co-mentor)

Abstract

V doktorskem delu smo proučevali vpliv nanomateriala črnega ogljika (NM CB) na membrane krvnih celic ter orjaške unilamelarne fosfolipidne mehurčke (OUFM). Glavni namen je bilo ugotoviti, ali dodani NM CB vpliva na biološke membrane, tako plazemske celične membrane kot tudi membrane OUFM. Izolirane krvne celice, polno kri ter OUFM smo inkubirali z NM CB v različnih koncentracijah. Opazovali smo spremembo oblike eritrocitov zaradi prisotnosti NM CB v vzorcih. Ugotovili smo statistično pomembne razlike v razmerju povprečnega števila diskocitov, ehinocitov in stomatocitov glede na kontrolne vzorce. Dodani NM CB je v vzorcih povzročil tudi adhezijo eritrocitov na aglomerate nanomateriala. Pri populaciji trombocitov smo opazili tubularne izrastke po 3 in 24 urah inkubacije tako v testnih kot kontrolnih vzorcih, zato aktivacije ne moremo pripisati dodanemu NM CB. Statistično pomembne razlike so se pojavile pri populaciji levkocitov, saj so testni vzorci vsebovali v povprečju povečano število levkocitov, eozinofilcev ter bazofilcev in znižano število monocitov glede na kontrolo. Inkubacija OUFM z NM CB je povzročila znatno in statistično pomembno zmanjšanje števila OUFM že po 20 minutah pri preostalih OUFM pa povečano povprečno ekscentričnost, glede na kontrolo. Ugotovili smo, da prisotnost NM CB v vzorcih s krvnimi celicami ni povzročila hemolize ali drugih sprememb, ki bi lahko bile bistvene za delovanje krvnih celic, medtem ko je prišlo do disintegracije membran OUFM, kar smo, tako kot spremembo oblike eritrocitov, pripisali predvsem sekundarnim osmotskim učinkom v raztopini.

Keywords

črni ogljik;nanomaterial;krvne celice;oblika eritrocitov;diskociti;stomatociti;ehinociti;trombociti;levkociti;orjaški unilamelarni fosfolipidni mehurčki;disertacije;

Data

Language: Slovenian
Year of publishing:
Typology: 2.08 - Doctoral Dissertation
Organization: UL ZF - University College of Health Studies
Publisher: [M. Pajnič]
UDC: 620.3:614.7:612.11(043.3)=163.6
COBISS: 935031 Link will open in a new window
Views: 1202
Downloads: 320
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Other data

Secondary language: English
Secondary title: Effect of carbon black nanomaterial on blood cell membranes
Secondary abstract: We investigated the effect of carbon black nanomaterial (NM CB) on membranes of blood cells and of giant unilamelar phospholipid vesicles (GUV). The aim of the study was to determine whether NM CB effects the shape of blood cells and GUVs. The samples of isolated blood cells, full human blood and GUVs were incubated with different concentrations of NM CB. We observed the shape changes of erythrocytes due to the presence of NM CB in the samples; the abundance of discocytes, echinocytes and stomatocytes in the treated samples was changed with respect to control samples. NM CB also affected the adhesion of erythrocytes to agglomerates of nanomaterial. Tubular protrusions on platelet membrane occurred after 3 and 24 hours both in the samples incubated with NM CB and in the control ones; no differences were found in activation of platelets in the respective samples. However, statistically significant differences were observed in the average number of leukocytes as well as of eosinophils, basophils and monocytes with respect to control samples. Incubation of GUVs with NM CB caused a considerable and statistically significant decrease in their number while the contours of the remaining GUVs exhibited on average higher eccentricity than the control ones. It was concluded that the presence of NM CB in the samples with blood cells did not cause hemolysis. We did not detect hemolysis or changes in cell membranes due to the presence of NM CB that could impair the function of blood cells. We observed disintegration of GUV membranes which was, similarly to the erythrocyte shape change, interpreted as a secondary osmotic effect.
Secondary keywords: carbon black;nanomaterial;blood cells;erythrocyte shape;discocytes;stomatocytes;echinocytes;platelets;leukocytes;giant unilamelar phospholipid vesicles;
Type (COBISS): Doctoral dissertation
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak.
Pages: XIII f., 70, [7] str.
ID: 11270702