Abstract
The aim of the present study was to characterize human milk microbiota (HMM) with 16S rRNA gene amplicon next-generation sequencing and cultivation/matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) identification approaches. We analyzed 31 human milk samples from healthy Slovenian mothers. To check the accuracy of MALDI-TOF MS identification, several colonies representing most abundant genera and those, which could not be reliably identified by MALDITOF, were subjected to Sanger sequencing of their 16S rRNA gene. We showed that cultivation/MALDI-TOF MS was a suitable tool for culture-dependent determination of HMM. With both approaches, Staphylococcus and Streptococcus were found as predominant genera in HMM and the abundance of Staphylococcus was associated with decreased microbial diversity. In addition, we characterized factors that might influence HMM. The use of a breast pump was significantly associated with composition of HMM, higher microbial load, and lower abundance of cultivable staphylococci. Moreover, our study suggests that administration of probiotics to the suckling infant might influence HMM by increased abundance of lactobacilli and the presence of viable probiotic bacteria in human milk. However, since our study was observational with relatively small sample size, more targeted studies are needed to study possible transfer of probiotics to the mammary gland via an external route and the physiological relevance of these events.
Keywords
mikrobiologija;humano mleko;mikrobiota;genetika;masna spektrometrija;
Data
Language: |
English |
Year of publishing: |
2019 |
Typology: |
1.01 - Original Scientific Article |
Organization: |
UL BF - Biotechnical Faculty |
UDC: |
579 |
COBISS: |
4326792
|
ISSN: |
1664-302X |
Views: |
1057 |
Downloads: |
855 |
Average score: |
0 (0 votes) |
Metadata: |
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Other data
Secondary language: |
Slovenian |
Secondary keywords: |
mikrobiologija;humano mleko;mikrobiota;genetika;masna spektrometrija; |
Type (COBISS): |
Article |
Source comment: |
Sistemske zahteve: Adobe Acrobat Reader |
System comment: |
Sistemske zahteve: Adobe Acrobat Reader |
Pages: |
str. 1-12 |
Volume: |
ǂVol. ǂ10 |
Issue: |
ǂart. ǂno. 2612 |
Chronology: |
2019 |
DOI: |
10.3389/fmicb.2019.02612 |
ID: |
11406153 |