magistrsko delo
Sabina Lazič (Author), Jana Murovec (Mentor)

Abstract

Protoplasti so rastlinske celice brez celične stene. Vsebino celice obdaja le celična membrana. So edinstven celični sistem, ki omogoča funkcionalne genetske analize in preučevanje širokega obsega celičnih procesov, kot so celična struktura, membransko delovanje, hormonska signalizacija. Izolacija protoplastov je biotehnološka tehnika pridobivanja protoplastov iz rastlinskega tkiva s pomočjo encimske razgradnje celične stene. Na uspešnost izolacije vplivajo različni dejavniki (fizikalni, fiziološki). Cilj magistrske naloge je bil preveriti uspešnost različnih postopkov izolacije protoplastov navadne konoplje (Cannabis sativa L.), med njimi izbrati uspešnega in ga optimizirati ter ohraniti njihovo živost. Ugotavljali smo s katero encimsko raztopino in iz katerih rastlinskih delov bomo izolirali največje število protoplastov. Rezultati so pokazali, da je iz mezofila listov in etioliranih hipokotilov možno izolirati največje število protoplastov. Pri mezofilnih listih je bila najuspešnejša nekaj urna inkubacija listov v encimski raztopini z 1,5 % celulazo Onozuka R-10 in 0,4 % macerocimom R-10. Pri etioliranih hipokotilih pa inkubacija preko noči v encimski raztopini z 1 % celulazo Onozuka R-10 in 0,1 % macerocimom R-10.

Keywords

izolacija;protoplast;encimska raztopina;navadna konoplja;Cannabis sativa;mezofil lista;etiolirani hipokotili;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [S. Lazič]
UDC: 582.630.1:577.1:602.6 (043.2)
COBISS: 12986627 Link will open in a new window
Views: 814
Downloads: 274
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Other data

Secondary language: English
Secondary title: Isolation of protoplasts from hemp (Cannabis sativa L.)
Secondary abstract: Protoplasts are plant cells lacking cell wall. Cell contents are surrounded by the cell membrane. They are a unique cell system to preform functional genetic analyses and studying wide specter of cell processes, for example cell structure, membrane function and hormonal signalization. Isolation of protoplast is a biotechnological technique for extracting protoplasts from plant tissue by enzymatic degradation of the cell wall. The success of isolation is influenced by various factors (physical, physiological ext.). The aim of the master's thesis was to check the success of protoplast isolation and viability of protoplasts of hemp (Cannabis sativa L.) by various procedures. We chose the most successful procedur and tried to optimize it. We determined which enzyme solution and from which plant parts the largest number of protoplasts were isolated. The results showed that the highest number of protoplasts can be isolated from mesophyll of leaf cells and etiolated hypocotyls. Few hours of incubation of leaves in enzyme solution composed of 1.5% Cellulase Onozuka R-10 and 0.4% of Macerozyme R–10, gave the best results. From etiolated hypocotyls the best results were obtained with enzyme solution composed of 1% Cellulase Onozuka R-10 and 0.1 % of Macerozyme R-10.
Secondary keywords: isolation;protoplast;enzymetic solution;hemp;mesophyll of leaf;etioltated hypocotyls;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za agronomijo
Pages: VIII, 38 str.
ID: 11587493