diplomsko delo
Rok Žiberna (Author), Kristina Sepčić (Mentor), Kristina Sepčić (Thesis defence commission member), Polona Jamnik (Thesis defence commission member), Nada Žnidaršič (Thesis defence commission member)

Abstract

Napredek raziskav na področju bakterijskih porotvornih toksinov (PFT) je v zadnjem desetletju razkril presenetljivo kompleksnost njihove zgradbe in sestave. Visoka strukturna raznolikost PFT in njihova sposobnost, da tvorijo selektivno in regulirano pot za vodo, ione in vodotopne molekule v bioloških in umetnih membranah, nudi številne možnosti za različne medicinske in biotehnološke aplikacije. Njihova uporabnost se kaže predvsem na področju detekcije in analize biomolekul. Pri tovrstni metodologiji gre za premestitev molekule (npr. DNA ali proteina) skozi poro, pri čemer se spremeni ena ali več lastnosti opazovanega sistema (membrana s poro). Spremembe se lahko z ustreznimi inštrumenti izmerijo in prevedejo na primer v zaporedje baznih parov ali v aminokislinsko zaporedje. Tovrstna tehnologija je na voljo za sekvenciranje DNA in se nenehno izboljšuje. Od prvotne uvedbe leta 2014 je pritegnila veliko zanimanja in si ustvarila veliko bazo uporabnikov. Trenutno je to edina komercialno dostopna aplikacija nanopor, vendar po količini novih člankov pisanih na to temo predvidevam, da se bo uporabnost razširila še na širši spekter analitov.

Keywords

porotvorni toksini;nanopore;aerolizin;citolizin A;sekvenciranje;detekcija biomolekul;analiza biomolekul;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [R. Žiberna]
UDC: 601.4:577.21(043.2)
COBISS: 23631363 Link will open in a new window
Views: 651
Downloads: 143
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Other data

Secondary language: English
Secondary title: Use of bacterial pore-forming toxins for the detection and analysis of biomolecules
Secondary abstract: Advances in research on bacterial pore-forming toxins (PFT) over the past decade have revealed surprising complexity in their structure and composition. The high diversity of PFT and their ability to generate selective and regulated pathways for water, ions and water-soluble molecules in biological in artificial membranes, makes them suitable for a wide variety of promising applications in biotechnology and medicine. Their applicability is mostly shown in biomolecule detection and analysis. This methodology involves the translocation of an analyte molecule (e.g. DNA or protein) through a nanopore, which results in alteration of one or more properties of the observed system (membrane with nanopore). These alterations can be measured with appropriate instruments and translated, for example, into a base pair sequence or amino acid sequence. This technology is currently available for DNA sequencing and is constantly improving. From its launch in 2014, the technology attracted a lot of interest and gathered a large user base. At the moment, this is the only commercially available nanopore application, but judging by the amount of recently published articles on this topic, it is suggestive that nanopore detection and analysis will further expand to a wider analyte range.
Secondary keywords: pore-forming toxins;nanopores;aerolysin;citolysin A;sequencing;biomolecule detection;biomolecule analysis;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 20 str.
ID: 11902820