diplomsko delo
Abstract
Pluripotentne matične celice so vir vseh celic, ki sestavljajo organizem. Med celicami v
pluripotentnem stanju pa obstajajo razlike. Zaenkrat je v literaturi opisanih več stanj
pluripotenosti, kot so: dvoceličnemu zarodku podobne celice, matične celice z razširjenim
potencialom, »extended« pluripotentne matične celice, naivne pluripotentne matične celice
in »primed« pluripotentne matične celice; vsako stanje ima svoje specifične lastnosti.
Glavne razlike so v izvoru, epigenetskih lastnostih, izraženih označevalcih in potencialu za
tvorbo himer. S preverjanjem sposobnosti tvorbe himer dobimo vpogled v njihov dejanski
potencial za diferenciacijo. Nekatera novo opisana stanja embrionalnih matičnih celic kažejo
določene značilnosti totipotentnih matičnih celic. Razlike se pojavljajo tudi v pogojih
pridobivanja in vzdrževanja različnih stanj pluripotentnih matičnih celic. S pogoji v kulturi
lahko vplivamo na njihovo stanje in funkcionalne lastnosti. Preko odkrivanja različnih
lastnosti spoznavamo pomen posameznih stanj ter delovanje in vlogo v razvoju zarodka.
Zanimiva je tudi primerjava različnih tipov pluripotentnih matičnih celic z in vivo stanjem
zarodka v različnih stopnjah razvoja. Razlike obstajajo tudi med matičnimi celicami
različnih vrst organizmov. Kljub številnim raziskavam je še veliko neznanega, določenih
stanj pluripotentosti pa trenutno še ne znamo ohranjati v in vitro pogojih.
Keywords
matične celice;pluripotentne matične celice;himerni test;DNA metilacija;kromosom X;gojišče;
Data
Language: |
Slovenian |
Year of publishing: |
2020 |
Typology: |
2.11 - Undergraduate Thesis |
Organization: |
UL BF - Biotechnical Faculty |
Publisher: |
[K. Smrkolj] |
UDC: |
602.9:591.81(043.2) |
COBISS: |
23618563
|
Views: |
602 |
Downloads: |
137 |
Average score: |
0 (0 votes) |
Metadata: |
|
Other data
Secondary language: |
English |
Secondary title: |
Comparison of different pluripotent states in cell cultures of stem cells |
Secondary abstract: |
Pluripotent stem cells are the origin of all cells that form an adult organism. Different states
of pluripotency with specific characteristics such as two-cell-like cells, expanded potential
stem cells, extended pluripotent stem cells, naïve pluripotent stem cells, and primed
pluripotent stem cells have been described. The main differences are the origin, epigenetic
characteristics, expressed markers, and the potential for chimera formation . By analysing
the ability of the chimera formation, we get an insight into their actual differentiation
potential. Some of the newly described types of embryonic stem cells show certain
characteristics of totipotent stem cells. The differences occur also in the conditions of
establishement and maintaining the different types of pluripotent stem cells. Type and
functional characteristics of pluripotent stem cells are culture conditions dependant. By
recognising different characteristics of embryonic stem cells will aid in discovering the role
and functions of different stem cell types duringearly embryonic development. Comparison
of different types of stem cells from the developing embryo with in vitro cultures of
embryonic stem cells is also intriguing. Significant differences are also observed between
stem cells of different organism. Despite intensive research many questions remain
unanswered and we are unable to grow some of the types of pluripotent cell in in vitro
conditions. |
Secondary keywords: |
stem cells;pluripotent stem cells;chimera assay;DNA methylation;X chromosome;medium; |
Type (COBISS): |
Bachelor thesis/paper |
Study programme: |
0 |
Embargo end date (OpenAIRE): |
1970-01-01 |
Thesis comment: |
Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije |
Pages: |
VIII, 22 str. |
ID: |
11902824 |