Jan Plut (Author), Urška Jamnikar (Author), Irena Golinar (Author), Tanja Knific (Author), Marina Štukelj (Author)

Abstract

Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of thevaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories andtested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected todirect Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences. Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all differentpig categories on five farms. A high to moderate correlation of strong statistical significance was found betweenindividual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to thatof other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On onefarm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did notaffect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, eachwith two control groups. Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed ahigh degree of similarity between Slovenian and GenBank isolates.

Keywords

viral disease;pig oral fluid;faeces;serum;PCV3;PCR;first report;Slovenia;Circoviridae infections - virology - veterinary;polymerse chain reaction - methods;phylogeny;genome viral - genetics;sequence analysis;DNA;swine;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL VF - Veterinary Faculty
UDC: 636.09:616
COBISS: 24828675 Link will open in a new window
ISSN: 1746-6148
Views: 572
Downloads: 198
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Other data

Secondary keywords: Virus Diseases;Serum;Polymerase Chain Reaction;Swine;
Type (COBISS): Article
Pages: str. 1-9
Volume: ǂVol. ǂ16
Issue: ǂart. no. ǂ281
Chronology: 2020
DOI: 10.1186/s12917-020-02489-y
ID: 11954086