Language: | Slovenian |
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Year of publishing: | 2020 |
Typology: | 2.09 - Master's Thesis |
Organization: | UM FKKT - Faculty of Chemistry and Chemical Engineering |
Publisher: | [A. Verdnik] |
UDC: | 66.061.34:[547.962.9:677.318](043.2) |
COBISS: | 32134659 |
Views: | 570 |
Downloads: | 40 |
Average score: | 0 (0 votes) |
Metadata: |
Secondary language: | English |
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Secondary title: | Isolation of keratin from waste wool using hydrothermal processes |
Secondary abstract: | The growing concern regarding the environment and the increasing demand for safe and sustainable biological materials are encouraging the search for better green methods of using available natural side products. Waste sheep wool with a high content of keratin (95 %) is just one of the side products that is created in the textile industry. It would be sensible to biodegrade the woollen keratin and transform it into commercial biomaterials which can be used in future applications as a secondary product. Through this the extraction of keratin from wastewater with sub-critical water represents an important alternative to the conventional processes of extraction. In the masters dissertation the keratin from the waste wool has been isolated by using sub-critical water. The isolation took place in a high-pressure and high-temperature reactor between the temperatures of 150 °C and 250 °C and a reaction time between 5 min to 75 min. The highest level of keratin was achieved at 180 °C and 60 min. with Fourjer’s transformation infrared spectroscopy we confirmed the presence of keratin in the obtained isolation products. The molecular mas of the obtained keratin was determined by using two different methods: with a polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE electrophoresis) and by using a gel-based chromatography. The molecular mass of keratin created by the isolation is between 4 kDa and 13 kDa. The molecular masses of keratin decrease with increasing of temperature. |
Secondary keywords: | waste wool;keratin;isolation;subcritical water;gel chromatography;electrophoresis; |
Type (COBISS): | Master's thesis/paper |
Embargo end date (OpenAIRE): | 2021-12-30 |
Thesis comment: | Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo |
Pages: | XII, 50 str. |
ID: | 11964834 |