diplomsko delo
Abstract
V diplomskem delu sem v vzorcih vina in vinskega kamna določevala naslednje fenolne spojine: 3,5-dihidroksibenzojsko kislino, p-kumarno kislino, vanilinsko kislino, galno kislino, sinapično kislino, (-)-epikatehin, elaginsko kislino, kvercetin in klorogensko kislino. Analizirala sem 3 vzorce vina: refošk, rumeni muškat in belo vino (neznane sorte) in 3 vzorce vinu pripadajočega vinskega kamna, iz katerega izvira. Določitev fenolnih spojin sem izvedla s tekočinsko kromatografijo visoke ločljivosti z UV/VIS detekcijo. Zaradi kompleksne sestave vzorcev sem te analizirala še s tekočinsko kromatografijo sklopljeno z masno spektrometrijo (MS), na podlagi katere sem izvedla tudi kvantitativno določitev.
Sklopitev z MS se je izkazala za primernejšo, saj sem s pomočjo te uspela določiti več fenolnih spojin, kot z UV/VIS detekcijo. Ugotovila sem, da so vzorci vina bolj bogati s fenolnimi spojinami, kot vinski kamen. V vseh vzorcih sem identificirala samo galno kislino, 3,5-dihidroksibenzojsko kislino in kvercetin; z izjemo belega vinskega kamna (VZ 2), ki je vseboval le kvercetin. Ostalih fenolnih spojin v nobenem od vzorcev nisem zaznala.
Keywords
vino;vinski kamen;fenolne spojine;analizne metode;tekočinska kromatografija visoke ločljivosti;HPLC;masna spektrometrija;MS;diplomska dela;
Data
Language: |
Slovenian |
Year of publishing: |
2020 |
Typology: |
2.11 - Undergraduate Thesis |
Organization: |
UL FKKT - Faculty of Chemistry and Chemical Technology |
Publisher: |
[M. Špendal] |
UDC: |
543.544.5HPLC:543.51(043.2) |
COBISS: |
26500867
|
Views: |
467 |
Downloads: |
127 |
Average score: |
0 (0 votes) |
Metadata: |
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Other data
Secondary language: |
English |
Secondary title: |
Determination of phenolic compounds in wine and in cream of tartar |
Secondary abstract: |
In my diploma thesis, the following phenolic compounds in wine and cream of tartar were determined: 3,5-dihydroxybenzoic acid, p-coumaric acid, vanillic acid, gallic acid, sinapic acid, (-)-epicatechin, ellagic acid, quercetin and chlorogenic acid. I analysed 3 wine samples: refošk, rumeni muškat and white wine (unknown varieties) and 3 samples of cream of tartar which originates from corresponding wine. The determination of phenolic compounds was performed by high-performance liquid chromatography with UV/VIS detection. Due to the complex composition of the samples, I analysed them by liquid chromatography, coupled with mass spectrometry (MS), based on which I also performed a quantitative determination.
Coupling with MS proved to be more appropriate as I was able to determine more phenolic compounds by this method than by UV/VIS detection. Wine samples were more enriched in phenolic compounds than cream of tartar. In all samples, only gallic acid, 3,5-dihydroxybenzoic acid and quercetin were identified, except for white cream of tartar (VZ 2) containing only quercetin. I did not detect any other phenolic compounds in any of the samples. |
Secondary keywords: |
wine;cream of tartar;phenolic compounds; |
Type (COBISS): |
Bachelor thesis/paper |
Study programme: |
1000373 |
Embargo end date (OpenAIRE): |
1970-01-01 |
Thesis comment: |
Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemija |
Pages: |
36 str. |
ID: |
11974435 |