diplomsko delo univerzitetnega študijskega programa I. stopnje
Gal Slaček (Author), Maša Knez Hrnčič (Mentor), Taja Žitek (Co-mentor), Andrej Golle (Co-mentor)

Abstract

V diplomskem delu smo raziskovali vpliv konvencionalnih ekstrakcijskih metod na vsebnost bioaktivnih komponent v brusnici. V laboratoriju smo izvajali ekstrakcijo po Soxhletu, hladno maceracijo, ultrazvočno ekstrakcijo in ekstarkcijo s superkritičnimi fluidi. Za pridobivanje željenih ekstraktov smo uporabili štiri topila (etanol, etanol+voda, voda in CO2), ki smo jih kasneje z rotacijskim uparjalnikom odparili. Ko smo vse željene ekstrakte pridobili smo pričeli z analiznim delom, kjer smo sprektrofotometrično določili vsebnosti antioksidantov, totalnih fenolov in proantocianidinov pri različnih valovnih dolžinah. Iz dobljenih meritev smo ugotovili, da imajo ekstrakti pridobljeni po metodi Soxhleta s topilom voda največjo antioksidativno aktivnost. Ekstrakti metode Soxhlet s topilom etanol in ekstrakti hladne maceracije kjer je topilo etanol+voda največjo vsebnost totalnih fenolov ter ekstrakti ultrazvočne ekstrakcije s topilom voda največji delež proantocianidinov. V drugem delu diplomske naloge smo ekstraktom, ki so nam dali najboljše rezultate določili še antimikrobni potencial. Izbrane ekstrakte smo nanašali na bakteriji Staphylococcus aureus, in Escherichia coli ter na glivo Candida albicans. Kot rezultat smo na mikrotitrskih ploščicah opazovali preskok barve, ki nam je predstavljal MIC.

Keywords

brusnica;ekstrakcije;antimikrobni potencial;antioksidanti;totalni fenoli;proantocianidini;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [G. Slaček]
UDC: 66.016.3:634.738(043.2)
COBISS: 34046723 Link will open in a new window
Views: 468
Downloads: 194
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: English
Secondary title: Effect of extraction method on the content of bioactive components of cranberry ( vaccinium vitis-idaea ) and antimicrobial potential
Secondary abstract: In the assay we investigated how different conventional extraction methods affect the content of bioactive components in cranberries. Soxhlet extraction, cold maceration, ultrasonic extraction and extraction with supercritical fluids were performed in the laboratory. Four solvents (ethanol, ethanol + water, water, and CO2) were used to obtain extracts, which were evaporated by rotary evaporation. Further, were made spectrophotometrically measurements to determined antioxidants, total phenols, and proanthocyanidins. Results showed that the extracts obtained by the Soxhlet method with a water solvent have the highest antioxidant activity. Soxhlet-extracts with the solvent ethanol and cold maceration-extracts with the solvent ethanol + water has the highest content of total phenols. Ultrasonic extracts with solvent water have the highest proportion of proanthocyanidins. In the second part of our work, were determined the antimicrobial potential of the extracts that gave the best results. Selected extracts were applied to Staphylococcus aureus, Escherichia coli, and Candida albicans. As a result, we determined a color leap on the microtiters plates, which represented the MIC.
Secondary keywords: cranberry;extractions;antimicrobial potential;antioxidants;total phenols;proanthocyanidins;
Type (COBISS): Bachelor thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: X, 21 str.
ID: 11975443