Enkapsulacija antibiotikov v porozne polimerne strukture

diplomsko delo univerzitetnega študijskega programa I. stopnje

Maja Gračner (Author), Maja Leitgeb (Mentor), Gregor Kravanja (Co-mentor), Mateja Primožič (Co-mentor)

Abstract

Porozni polimerni materiali se čedalje bolj pojavljajo kot predmet preučevanja v različnih študijah, saj kažejo velik potencial pri nadzorovanem sproščanju zdravil in v drugih biomedicinskih aplikacijah. Predvsem se proučujejo lastnosti hidrogelov iz biorazgradljivih polimerov, ki so primerni za uporabo v človeškem organizmu. V diplomski nalogi smo poskusili razviti pH senzitivni porozni material, hidrogel iz biorazgradljivih polimerov za kontrolirano sproščanje antibiotikov. Sintetizirali smo dva različna hidrogela oz. polimerne mreže, ju posušili s pomočjo liofilizacije in proučevali njune lastnosti z različnimi metodami. Nastale polprepustne polimerne mreže (semi-IPN) smo pridobili iz hitozana in mešanice hitozana ter alginata (50:50) s polivinil pirolidonom (PVP) in glutaraldehidom. Vezi znotraj hidrogela smo okarakterizirali s pomočjo Fourierjeve infrardeče spektroskopije (FTIR). Porozna materiala s hitozanom ter s hitozanom/alginatom smo med sabo primerjali glede na površinsko morfologijo (ESEM analiza), kinetiko nabrekanja odvisno od pH, degradacijo in delež topnosti, kontaktni kot ter poroznost. V drugem koraku smo med sintezo hidrogelov homogeno dodali še aktivno učinkovino ciprofloksacin. Proučevali smo sproščanje antibiotika v odvisnosti od časa ter primerjali rezultate obeh hidrogelov. Liofilizirani hidrogeli z visoko poroznostjo so pokazali velik vpliv pH na lastnosti nabrekanja, nizki kontaktni koti pa na hidrofilno naravo hidrogelov. Rezultati so v kislih pogojih pokazali povečan delež nabrekanja, kar lahko pripišemo protonaciji primarne amino skupine hitozana in karboksilne skupine alginata. Pri sproščanju smo najboljše pogoje zaznali pri pH 1,5, kjer se je sprostilo okrog 55 % ciprofloksacina v 2 urah. Liofilizirani hidrogeli iz hitozana/alginata so pokazali malce boljše rezultate pri sproščanju, vendar bi v prihodnosti oba lahko služila kot potencialna kandidata za dostavo aktivnih učinkovin v kislih pogojih.

Keywords

hidrogel;pH-senzitiven;liofilizacija;hitozan;alginat;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2020
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[M. Gračner]
UDC:	544.773.432.057(043.2)
COBISS:	30018819
Views:	422
Downloads:	101
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Encapsulation of antibiotics into porous polymer structures
Secondary abstract:	Porous polymeric materials are increasingly being studied in various studies, as they show great potential in the controlled release of drugs and in other biomedical applications. In particular, the properties of hydrogels from biodegradable polymers suitable for use in the human organism are being studied. In our thesis we tried to develop pH sensible porous material, hydrogel from biodegradable polymers for controlled antibiotic release. We synthesized two different hydrogels or polymer nets, dried them with the help of lyophilization and studied their properties by different methods. Semi-permeable polymer nets (semi-IPN) were obtained from chitosan and a mixture of chitosan and alginate (50:50) with polyvinyl pyrrolidone (PVP) and glutaraldehyde. The bindings inside the hydrogel were checked using Fourier transform infrared spectroscopy (FTIR). Porous material with chitosan and chitosan/alginate was compared according to surface morphology (ESEM analysis), swell kinetics depending on pH, degradation and solubility rate, contact angle and porosity. In the second step, the active substance ciprofloxacin was added homogeneously during the synthesis of hydrogels. We studied the release of the antibiotic over time and compared the results of the two hydrogels. Lyophilized high porosity hydrogels showed a significant influence of pH on the properties of swelling and low contact angles on the hydrophilic nature of hydrogels. The results showed an increased rate of swelling in acidic conditions, which can be attributed to protonation of the primary amino group of chitosan and carboxylic of alginate. When released, the best conditions were detected at pH 1.5, where about 55 % of ciprofloxacin was released within 2 hours. Lyophilized hydrogels from chitosan/alginate have shown slightly better release results, but in the future, both could serve as potential candidates for delivery of active substances in acidic conditions.
Secondary keywords:	hydrogel;pH-sensitive;lyophilization;chitosan;alginate;
Type (COBISS):	Bachelor thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	XII, 58 str.
ID:	11975565