Vsebnost ftalatov v biološkem mediju

diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje

Živa Nekrep (Author), Petra Kotnik (Mentor), Maša Knez Hrnčič (Co-mentor)

Abstract

Ftalati so estri 1,2-benzendikarboksilne kisline. Produkti, proizvedeni iz ftalatov, so najpogosteje mehčalci, ki izboljšajo fleksibilnost in uporabnost polimerov z visoko molekulsko maso. Linearni in razvejani ftalatni estri so uporabljeni v proizvodnji plastike. Najdemo jih v črnilih, barvi, lepilih, talnih oblogah, nekaterih živilskih proizvodih, kozmetiki in farmacevtskih produktih. Spadajo med endokrine motilce, ker vplivajo na normalno hormonsko delovanje v človeškem telesu. Izpostavljenost določenim ftalatom lahko med drugim povzroči anomalije v razvoju ploda med nosečnostjo, preeklampsijo in prezgodnji porod, povezana je tudi s težavami v nevrološkem razvoju, z nižjo kvaliteto semenčic, s splavom, z astmo in alergijami ter debelostjo. Ljudje smo jim lahko izpostavljeni z inhalacijo, absorpcijo skozi kožo ter zaužitjem. Absorpcija, metabolizem, distribucija in eliminacija kemikalij so odvisni od načina vstopa v telo. V diplomski nalogi smo razvili učinkovito in kratko metodo z zadovoljivimi rezultati za določanje dietil in dibutil ftalata v krvnem serumu in urinu. Dietil ftalat se nahaja v izdelkih za osebno nego (dišave), v prevlekah tablet v farmaciji, barvah in insekticidih. Dibutil ftalat uporabljajo pri prizvodnji celulozno acetatne plastike, v izdelkih za osebno nego (lak za nohte, kozmetika), laku in prevlekah tablet. Za ekstrakcijo omenjenih ftalatov iz bioloških vzorcev smo uporabili ekstrakcijo tekoče-tekoče in za določitev plinsko kromatografijo z masno spektrometrijo (GC-MS).

Keywords

ftalati;dibutil ftalat;dietil ftalat;ekstrakcija;biološki vzorci;urin;krvni serum;plinska kromatografija z masno spektrometrijo;diplomske naloge;

Data

Language:	Slovenian
Year of publishing:	2020
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[Ž. Nekrep]
UDC:	661.8'078.4:638.16(043.2)
COBISS:	32464643
Views:	475
Downloads:	72
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Phthalate content in biological matrix
Secondary abstract:	Phthalates are esters of 1,2-benzenedikarboxylic acid. The products, produced from phthalates, are most commonly softeners, that improve flexibility and usability of polymers with high molecular mass. Linear and branched phthalate esters are used in production of plastics. We can find them in inks, colors, glues, flooring, animal products, cosmetics and pharmaceutical products. Phthalates are endocrine disruptors, beacuse they affect on normal hormonal activity in human body. Exposure to some pthalates can among other things cause anomalies in development of fetus during pregnancy, preeclampsia and early labour, it is also conected with problems in neurological development, lower semen quality, abortion, asthma, allergy and obesity. Humans can be exposed to them with inhalation, absorption through skin and ingestion. Absorption, metabolism, distribution and elimination of chemicals are dependent on their entrance into body. In diploma thesis, we have developed an effective and short method with adequate results for determination of diethyl and dibutyl phthalate in blood serum and urine. Diethyl phtalate is commonly found in products fot personal care (fragrances), tablet coatings in pharmacy, colors and insecticides. Dibutyl phtalate is used in cellulose-acetate plastics, products for personal care (polish, cosmetics), varnish and tablet coatings. For extraction of aforementioned phthalates from biological matrices we used liquid-liquid extraction and for determination gas chromatography with mass spectrometry (GC-MS).
Secondary keywords:	phthalates;dibutyl phtalate;dietil phthalate;extraction;biological samples;urine;blood serum;gas chromatography with mass spectrometry;
Type (COBISS):	Bachelor thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	XII, 42 str.
ID:	11988569