diplomsko delo univerzitetnega študijskega programa I. stopnje
Mojca Hraš (Author), Mojca Škerget (Mentor), Majda Hadolin Kolar (Co-mentor)

Abstract

Okoli 19. stoletja je navadni divji kostanj (Aesculus hippocastanum L.) postal cenjena zdravilna rastlina. Gre za drevo, ki ga zaradi njegovega uspešnega prilagajanja na različne tipe podnebij, najdemo skoraj povsod po svetu. Skozi leta so spoznavali njegove zdravilne učinke in ugotovili, da ima glavne farmakološke lastnosti aescin, mešanica triterpenskih saponinov, ki je prisoten v semenih navadnega divjega kostanja. Naš namen je bil preučiti pogoje pri katerih izoliramo največ aescina iz semen. Izolacijo smo izvedli z ekstrakcijo oz. natančneje maceracijo. Kot topilo smo uporabili 50 in 60 vol% etanol. Ekstrakcijo smo izvedli pri 40 in 60 °C, čas ekstrakcije pa je bil 2 ali 4 h. Ugotovili smo, da pri višji temperaturi in večjem deležu etanola v topilu, dobimo višjo vsebnost aescina v ekstraktu. Delež aescina v ekstraktu pa se je zmanjševal s časom ekstrakcije. Vsebnost učinkovine, aescina, v izoliranih ekstraktih smo analizirali z metodo iz francoske farmakopeje, ki temelji na spektrofotometrometriji. Metode v podjetju Vitiva d.d., kjer smo izvajali eksperimentalni del diplomske naloge, do sedaj še niso validirali. Zato smo izvedli tudi validacijo, kjer smo dokazovali selektivnost, linearnost in ponovljivost, kamor uvrščamo še ponovljivost metode, ponovljivost sistema ter vmesno natačnost in obnovljivost. Metodo smo v sklopu diplomske naloge uspešno validirali.

Keywords

divji kostanj;ekstrakcija;spektrofotometrija;validacija;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [M. Hraš]
UDC: 66.061.34(043.2)
COBISS: 33275907 Link will open in a new window
Views: 690
Downloads: 121
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Other data

Secondary language: English
Secondary title: Optimization of extraction and validation of analytical method of aescin in horse-chestnut extract
Secondary abstract: Around the 19th century, the common horse chestnut (Aesculus hippocastanum L.) became a valued medicinal plant. It is a tree that is found almost everywhere in the world due to its successful adaptation to different types of climates. Over the years its healing effects were discovered and found that the main pharmacological effects are generated by aescin, a mixture of triterpene saponins hidden in the seeds of the common horse chestnut. The aim of this work was to study conditions by which the most aescin is isolated from seeds. Isolatio was performed with extraction or more precisely maceration. As a solvent 50 and 60 vol% ethanol was used. Extraction was performed at 40 and 60 ° C while the extraction time was 2 or 4 h. It was found that the higher the temperature and the higher the ethanol content in the solvent is, the higher is the aescin content in the extract. The proportion of aescin in the extract, however, decreased with the extraction time. The content of the active substance-aescin in the isolated extracts was analyzed by a method from the French Pharmacopoeia that is based on spectrophotometrometry. The analytical method in the company Vitiva d.d., where we performed the experimental part of the diploma thesis, have not yet been validated. Therefore, we also performed validation, where we proved: selectivity, linearity and repeatability, which also includes repeatability of the method, repeatability of the system and intermediate accuracy and reproducibility. The method was successfully validated in the frame of the diploma thesis.
Secondary keywords: horse chestnut;aescin;extraction;spectrophotometry;validation;
Type (COBISS): Bachelor thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: X, 60 str.
ID: 11991730