diplomsko delo
Neja Kavčič (Author), Aleš Podgornik (Mentor)

Abstract

Kromatografija je laboratorijska tehnika pri kateri ločujemo posamezne komponente zmesi glede na njihovo separacijsko hitrost. Zmes je raztopljena v tako imenovani mobilni fazi, ki nato potuje skozi stacionarno fazo. Določevanje identitete in koncentracije molekul v zmesi izvajamo pri analitični kromatografiji. Preparativna kromatografija pa se uporablja za pripravo večjih količin čistih snovi posamezne molekulske vrste, ki jih lahko uporabimo kasneje. Virusi in virusom podobni delci (molekule, ki spominjajo na viruse, vendar so ne infektivne, ker ne vsebujejo genetskega materiala) ter bakteriofagi spadajo med nanoplekse in obdelava le teh je odvisna od kompleksnosti začetnega materiala, števila načinov očiščenja med katerimi izbiramo ter kriterijev, katerim mora končni produkt slediti. Prečiščeni virusni pripravki se že dolgo uporabljajo kot cepiva in imajo v prihodnosti veliki potencial v genski terapiji. Bakterijski virusi oziroma bakteriofagi že dokazano lahko zdravijo bakterijske okužbe, vendar je odkrivanje antibiotikov upočasnilo raziskavo v zvezi z njihovo uporabo. Zaradi večje odpornosti bakterij na antibiotike pa so spet prepoznani kot alternativni vir zdravljenja bakterijskih okužb. Prav zaradi tega bomo v naši raziskavi preučili možnost uporabe kromatografije za izolacijo virusov.

Keywords

virologija;virusi;virusom podobni delci;čiščenje virusov;gelska izključitvena kromatografija;ionsko izmenjevalna kromatografija;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [N. Kavčič]
UDC: 66.081.3:578(043.2)
COBISS: 29649667 Link will open in a new window
Views: 411
Downloads: 105
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Other data

Secondary language: English
Secondary title: Chromatography of viruses and virus-like particles
Secondary abstract: Chromatography is a laboratory technique where individual components of a mixture are separated according to their separation rate. The mixture is dissolved in the so-called mobile phase, which then travels through the stationary phase. The determination of the identity and concentration of molecules in the mixture is performed by analytical chromatography. Preparative chromatography, however, is used to prepare large quantities of pure substances of each molecular species that can be used later. Viruses and virus-like particles (molecules that resemble viruses but are non-infectious because they do not contain genetic material) and bacteriophages are nanoplexes and their processing depends on the complexity of the starting material, the number of purification methods to choose from, and the criteria which the end product has to match. Purified viral preparations have already been used as vaccines and have potential in gene therapy. Bacterial viruses or bacteriophages have already been proven to treat bacterial infections, but antibiotics have slowed researches regarding them. Due to the higher resistance of bacteria to antibiotics, they are again recognized as an alternative source of treatment for bacterial infections. It is for this reason that our study will examine the use of chromatography for virus isolation.
Secondary keywords: viruses;virus like particles;chromatography;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000372
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemijsko inženirstvo
Pages: 24 str.
ID: 12031213