diplomsko delo
Veronika Bukvič (Author), Borut Bohanec (Mentor), Borut Bohanec (Thesis defence commission member), Polona Jamnik (Thesis defence commission member), Mojca Narat (Thesis defence commission member)

Abstract

Čeprav so rastlinski virusi pomemben del ekosistema, povzročajo izgubo pridelka in škodujejo kmetijskim rastlinam, ki so ključnega pomena za preživetje ljudi in živali. S klasičnimi in novejšimi metodami pridobivamo izboljšane lastnosti rastlin in spoznavamo mehanizme interakcij med gostiteljem in virusom. Z razvojem tehnik genomskega preurejanja kot je CRISPR/Cas sistem, so se odprle možnosti poseganja v genom in še boljšega razumevanja funkcij določenih genov in molekul. Namen dela je povzeti možnosti aplikacij CRISPR/Cas sistema za pridobitev odpornosti na DNA in RNA viruse v kmetijskih rastlinah. Rezultati študij kažejo, da je ta tehnika natančna in uspešna pri pridobitvi odpornosti na viruse kmetijskih rastlin kot so paradižnik, krompir, pšenica, ječmen, manioka, bananovec in pesa. Analize so pokazale, da stopnja odpornosti na viruse korelira s stopnjo izražanja sgRNA molekule in Cas proteina. Optimizacija postopkov je ključna za uspešen vnos CRISPR/Cas sistema in učinkovitost pridobljene odpornosti. Omejitve v razvoju in komercializaciji te metode pa predstavljajo etični vidiki in stroga regulacija na področju GSO.

Keywords

rastlinski virusi;genomsko preurejanje;CRISPR/Cas sistem;rezistenca na viruse;kmetijske rastline;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [V. Bukvič]
UDC: 602.6:582.5/.9:606:631.528:632(043.2)
COBISS: 31116291 Link will open in a new window
Views: 405
Downloads: 83
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Other data

Secondary language: English
Secondary title: Use of genome editing methods to gain viral immunity in crops
Secondary abstract: Even though plant viruses are an important part of our ecosystem, they cause enormous yield losses and are hurting crops that are essential for survival of humans and animals. With classical and new methods we are gaining improved quality in plants and are exploring mechanisms of interactions between host and virus. Development of new genome editing techniques like the CRISPR/Cas system opened new possibilities to interfere with the genome and helped us to better understand functions of certain genes and molecules. Purpose of this work is to sum up potential application of CRISPR/Cas system to gain resistance for DNA and RNA viruses in crops. Results of the studies show that this technique is precise and efficient in gaining viral immunity of crops like tomato, potato, wheat, barley, cassava, banana and beet. The analysis shows that the level of resistance is correlated with level of sgRNA molecule and Cas protein expression. Optimisation of procedures is vital for successful delivery of CRISPR/Cas system and efficiency of gained resistance. Limitations in development and commercialisation of this method are aspects of ethical matters and strict GMO regulations.
Secondary keywords: plant viruses;genome editing;CRISPR/Cas system;viral resistance;crops;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: VI, 22 str.
ID: 12033059