diplomsko delo - univerzitetni študij - 1. stopnja
Matija Kadunc (Author), Tine Hauptman (Mentor), Barbara Piškur (Co-mentor)

Abstract

V diplomskem delu smo proučevali vpliv glive Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya na mali jesen (Fraxinus ornus L.). Zanimalo nas je, ali gliva povzroča poškodbe, značilne za jesenov ožig, tudi na malem jesenu. Prisotnost glive na malem jesenu smo ugotavljali na dva načina, in sicer s preverjanjem prisotnosti apotecijev glive H. fraxineus na odpadlih listnih pecljih in z izolacijo gliv iz nekrotičnih poganjkov. Terenski del raziskave smo izvajali na petih rastiščih malega jesena v Zasavski regiji. Prisotnost apotecijev v opadu na teh lokacijah smo preverjali 24. 6. 2020 in 26. 6. 2020. Apotecijev glive nismo odkrili, zato smo na vsaki lokaciji nabrali 30 odpadlih pecljev malega jesena in jih nato inkubirali na vlažnem papirju v steklenih petrijevkah. Spremljanje razvoja apotecijev je potekalo od 28. 6. 2020 do 7. 8. 2020 pri temperaturah med 22 % in 24 %. Apoteciji se na pecljih niso razvili. Izolacije gliv smo izvedli iz sedmih vzorcev nekrotičnih poganjkov malega jesena ter treh kontrolnih vzorcih velikega jesena (Fraxinus excelsior L.). Skupno smo pridobili sedem različnih morfotipov gliv. Molekularna analiza pridobljenih morfotipov je pokazala, da gre za glive Pezicula sp., Cytospora sp., Eutypa sp., Eutypa lata, Phomopsis sp., Didymella macrostoma ter Biscogniauxia nummularia. Glive H. fraxineus iz odvzetih vzorcev nismo uspeli izolirati. Prišli smo do zaključka, da imata najboljši potencial za povzročanje poškodb na malem jesenu glivi Phomopsis sp. in Biscogniauxia nummularia. Kljub temu, da prisotnosti glive H. fraxineus na malem jesenu nismo potrdili, ne moremo trditi, da gliva poškodb malega jesena na izbranih lokacijah ni povzročila.

Keywords

Hymenoscyphus fraxineus;jesenov ožig;mali jesen;Fraxinus ornus L.;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [M. Kadunc]
UDC: 630*44:630*17(Fraxinus ornus L.)(043.2)=163.6
COBISS: 32123395 Link will open in a new window
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Other data

Secondary language: English
Secondary title: Ash dieback (Hymenoscyphus fraxineus) on Flowering ash (Fraxinus ornus L.)
Secondary abstract: We studied the influence of the fungus Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya on flowering ash (Fraxinus ornus L.). We were interested in whether the fungus causes damage typical of ash dieback on flowering ash. The presence of the fungus in flowering ash was checked in two different ways, by checking the presence of apothecia of the fungus H. fraxineus on fallen leaf petioles and by isolating fungi from necrotic shoots. The field research was carried out on five sites of flowering ash in Zasavje. The presence of apothecia in leaf litter was checked on 24th and 26th of June 2020 at these locations. We did not detect any apothecia, so we collected 30 petioles of flowering ash in the leaf litter at each location and then incubate them on wet paper in glass petri dishes. Monitoring of apothecia development was performed from 28. 6. 2020 to 7. 8. 2020 at temperatures between 22 % and 24 %. Apothecia did not develop on the leaf petioles. Fungi isolations were performed from seven samples of necrotic shoots of flowering ash and from three control samples of common ash (Fraxinus excelsior L.). In total, we obtained seven different fungal morphotypes. Molecular analysis of the obtained morphotypes showed the following fungi species: Pezicula sp., Cytospora sp., Eutypa sp., Eutypa lata, Phomopsis sp., Didymella macrostoma and Biscogniauxia nummularia. Isolation of H. fraxineus from the obtained fungi isolates was unsuccessful. We concluded that the fungi with the best potential to cause damage on flowering ash were Phomopsis sp. and Biscogniauxia nummularia. Although the presence of H. fraxineus on flowering ash was not confirmed, we cannot conclude that the fungus did not cause damage to the flowering ash in selected locations.
Secondary keywords: Hymenoscyphus fraxineus;ash dieback;flowering ash;Fraxinus ornus L.;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za gozdarstvo in obnovljive gozdne vire
Pages: VIII, 40 f., [3] f. pril.
ID: 12044697