Language: | Slovenian |
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Year of publishing: | 2013 |
Source: | Ljubljana |
Typology: | 2.11 - Undergraduate Thesis |
Organization: | UL FFA - Faculty of Pharmacy |
Publisher: | [K. Masilo] |
UDC: | 543+547.96(043.2) |
COBISS: | 3395697 |
Views: | 1100 |
Downloads: | 258 |
Average score: | 0 (0 votes) |
Metadata: |
Secondary language: | English |
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Secondary title: | Selection of ligands of staphylococcal protein A from phage-displayed peptide libraries |
Secondary abstract: | Staphylococcal protein A is a surface protein of Staphylococcus aureus which binds human and animal antibody constant region to prevent adaptive immune response of the host. Due to effective mechanisms of evading the immune system and resistance to antibiotics, scientists are searching new ways of treating staphylococcal infections. One of possible strategies is protein A targeted drug delivery. Protein A is also widely used in biotechnology as an immunochemical reagent for antibody identification and purification. The aim of this research was to isolate peptide ligands with affinity to streptavidin and protein A from phage-displayed peptide libraries with affinity selection. With test affinity selection on streptavidin we isolated clones with typical motif HPQ and high affinity to streptavidin. With affinity selection on protein A from library Ph.D.-C7C™ with non-specific elution we isolated a peptide CAKTDPRGC and confirmed its binding to protein A with quantitative real-time polymerase chain reaction. The peptide's sequence is very similar to the sequence of antibody constant region, which is natural ligand of protein A. The selection from library Ph.D.-C7C™ with specific elution was not successful. We isolated peptides with distinct motif GKL with specific elution with antibody constant region from library Ph.D.-12™. Peptides GGKLTTIHLHST and GISDFMQTKMPL specifically bind to protein A, which was confirmed with quantitative real-time polymerase chain reaction. |
Secondary keywords: | Staphylococcal protein A Staphylococcus aureus phage-display protein A ligand affinity selection; |
Type (COBISS): | Undergraduate thesis |
Thesis comment: | Univ. v Ljubljani, Fakulteta za farmacijo |
Pages: | 60 str. |
ID: | 12053295 |