diplomsko delo
Jaka Krušič (Author), Matevž Pompe (Mentor)

Abstract

Za analizo ogljikovih hidratov je danes v uporabi mnogo različnih metod, v industrijski rabi je večina kromatografskih. V diplomskem delu opisujem kromatografsko določitev ogljikovih hidratov s fluorescenčno detekcijo. V sklopu tega sem optimiziral gradient za ločbo mešanice ogljikovih hidratov, primerjal med sabo tri različna barvila za fluorescenčno določitev ogljikovih hidratov in preveril obstojnost derivatov na sobni temperaturi in v zmrzovalniku. Za končno metodo sem izvedel tudi validacijo, kar je vključevalo preverjanje ponovljivosti, linearnosti in pa določitev meje detekcije in kvantifikacije.

Keywords

ogljikovi hidrati;analizne metode;tekočinska kromatografija hidrofilnih interakcij;HILIC;fluorescenčna detekcija;2-aminobenzojska kislina;2-AA;2-aminobenzamid;2-AB;2-aminopiridin;2-AP;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [J. Krušič]
UDC: 543.635.2(043.2)
COBISS: 36761859 Link will open in a new window
Views: 357
Downloads: 80
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Other data

Secondary language: English
Secondary title: Determination of carbohydrates using fluorescence detection
Secondary abstract: There are many different analytical procedures for carbohydrate determination, most of them used in the industry are chromatographic. In this diploma thesis I discuss chromatographic determination of carbohydrates with fluorescence detection. As part of this thesis I optimized the mobile phase gradient for the separation of a carbohydrate mixture, I compared three different labels used for carbohydrate detection with fluorescence and I checked the stability of the derivatives at room temperature and in a freezer. I also performed validation of the final method, which included checking of repeatability, linearity and determination of the limit of detection and quantification.
Secondary keywords: carbohydrates;fluorescence detection;liquid chromatography;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000373
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemija
Pages: 40 str.
ID: 12057198